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Literature summary for 3.2.1.154 extracted from

  • Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.
    Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.) (2003), Plant J., 36, 697-710.
    View publication on PubMed
Search for more literature for 3.2.1.154

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Pichia pastoris Beta vulgaris

Inhibitors

Inhibitors Comment Organism Structure
sucrose
-
 Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
77
-
 6-kestose
-
 Beta vulgaris

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
 cv. Opus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Beta vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Beta vulgaris
-
petiole
-
 Beta vulgaris
-
root
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.5
-
-
 Beta vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O native enzyme shows 12% of the activity with levan Beta vulgaris ?
-
 ?
6-kestose + H2O native enzyme shows 77% of the activity with levan Beta vulgaris ?
-
 ?
levan + H2O
-
 Beta vulgaris ?
-
 ?
levanbiose + H2O native enzyme shows 63% of the activity with levan Beta vulgaris beta-D-fructose
-
 ?
additional information no activity with sucrose, 1,1-nystose and inulin Beta vulgaris ?
-
 ?
neokestin + H2O native enzyme shows 25% of the activity with levan Beta vulgaris ?
-
 ?
neokestose + H2O native enzyme shows 93% of the activity with levan Beta vulgaris ?
-
 ?
phlein + H2O native enzyme shows 84% of the activity with levan Beta vulgaris ?
-
 ?

Synonyms

Synonyms Comment Organism
6-FEH
-
 Beta vulgaris
fructan 6-exohydrolase
-
 Beta vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Beta vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 38 18°C: about 45% of maximal activity, 38°C: about 20% of maximal activity Beta vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Beta vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6.8 pH 4.3: about 65% of maximal activity, pH 6.8: about 40% of maximal activity Beta vulgaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
134
-
 sucrose
-
 Beta vulgaris