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Literature summary for 3.2.1.152 extracted from

  • Ishimizu, T.; Hashimoto, C.; Kajihara, R.; Hase, S.
    A retaining endo-{beta}-mannosidase from a dicot plant, cabbage (2006), J. Biochem., 139, 1035-1043.
    View publication on PubMed
Search for more literature for 3.2.1.152

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside in ammonium acetate buffer, pH 5.0, at 37°C Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration Brassica oleracea
28000
-
 1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration Brassica oleracea
34000
-
 1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration Brassica oleracea
42000
-
 1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration Brassica oleracea
141000
-
 SDS-PAGE Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica oleracea
ammonium sulfate precipitation, Poros HS column chromatography, hydroxyapatite chromatography, DEAE-Sephacel column chromatography, Octyl Sepharose CL-4B column chromatography, and Superdex 200 gel filtration Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man-alpha(1-6)[Man-alpha(1-3)]Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O
-
 Brassica oleracea Man-alpha(1-6)[Man-alpha(1-3)]Man-alpha-(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide
-
 ?
Man-alpha-(1-6)Man-alpha(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O
-
 Brassica oleracea Man-alpha-(1-6)Man-alpha(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide
-
 ?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O
-
 Brassica oleracea Man-alpha-(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide
-
 ?
Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside 40% activity relative to M2B-PA Brassica oleracea Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
Manalpha(1-3)[Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O 34% activity relative to Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside Brassica oleracea Manalpha(1-3)[Manalpha(1-6)]Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O 100% activity Brassica oleracea Manalpha(1-6)Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O 2.6% activity relative to M2B-PA Brassica oleracea beta-D-mannose + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
additional information Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-3)[Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[(Manalpha(1-3))]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, GlcNAcbeta(1-4)Manalpha(1-3)[GlyNAcbeta(1-4)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-6)[Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))]GlcNAc-pyridylaminoside, p-nitrophenyl alpha-mannoside, p-nitrophenyl beta-mannoside, and Manbeta-(1-4)Manbeta(1-4)Man-beta-(1-4)Manbeta(1-4)Man-beta(1-4)Man are no substrates Brassica oleracea ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration Brassica oleracea

Synonyms

Synonyms Comment Organism
endo-beta-mannosidase
-
 Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Brassica oleracea

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 relatively stable at pH 3-6 Brassica oleracea