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Literature summary for 3.2.1.151 extracted from

  • Ishida, T.; Yaoi, K.; Hiyoshi, A.; Igarashi, K.; Samejima, M.
    Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium (2007), FEBS J., 274, 5727-5736.
    View publication on PubMed
Search for more literature for 3.2.1.151

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris, with and without the C-terminal carbohydratebinding module domain Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value is 0.25 mg/ml for enzyme with C-terminal carbohydrate binding module domain, and 0.28 mg/ml for enzyme without C-terminal carbohydratebinding module domain Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE, enzyme without C-terminal carbohydratebinding module domain, x * 130000, SDS-PAGE, enzyme with C-terminal carbohydratebinding module domain Phanerodontia chrysosporium
130000
-
 x * 80000, SDS-PAGE, enzyme without C-terminal carbohydratebinding module domain, x * 130000, SDS-PAGE, enzyme with C-terminal carbohydratebinding module domain Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein possibly both N- and O-glycosylation Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information very low activity against carboxymethyl cellulose, phosphoric acid-swollen cellulose, avicel, glucomannan, galactomannan, and xylan Phanerodontia chrysosporium ?
-
 ?
xyloglucan + H2O source of substrate: tamarind seed Phanerodontia chrysosporium xyloglucan oligosaccharides hydrolysis of unbranched glucose residues. Enzyme initially produces oligosaccharides with a degree of polymerization of 16-18, which are slowly hydrolyzed to final products with a degree of polymerization of 7-9. The ratio of the oligosaccharides with 7-9 to 16-18 glucose units is additionally dependent upon the pH value ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE, enzyme without C-terminal carbohydratebinding module domain, x * 130000, SDS-PAGE, enzyme with C-terminal carbohydratebinding module domain Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.1
-
 xyloglucan 30°C, pH 5, enzyme with C-terminal carbohydrate binding module domain Phanerodontia chrysosporium
31.9
-
 xyloglucan 30°C, pH 5, enzyme without C-terminal carbohydrate binding module domain Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 enzyme initially produces oligosaccharides with a degree of polymerization of 16-18, which are slowly hydrolyzed to final products with a degree of polymerization of 7-9. The ratio of the oligosaccharides with 7-9 to 16-18 glucose units is additionally dependent upon the pH value Phanerodontia chrysosporium