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Literature summary for 3.2.1.151 extracted from
- Specific xyloglucanases as a new class of polysaccharide-degrading enzymes (2004), Biochim. Biophys. Acta, 1674, 268-281.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-value for xyloglucan is 0.67 mg/ml | Aspergillus japonicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Aspergillus japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus japonicus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with beta-glucan and carboxymethylcellulose | Aspergillus japonicus | ? | - |
? |  |
| xyloglucan + H2O | high specific activity towards tamarind xyloglucan | Aspergillus japonicus | xyloglucan oligosaccharides | XXXG, XXLG and XLLG oligosaccharides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Aspergillus japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 5.0, stable for at least 24 h | Aspergillus japonicus |
| 50 | - |
half-life: 24 h | Aspergillus japonicus |
| 60 | - |
28 h, 50% loss of activity | Aspergillus japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53 | - |
xyloglucan | - |
Aspergillus japonicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 6.1 | more than 50% of maximal activity in the pH-range 3.8-6.1 | Aspergillus japonicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
40°C, stable for at least 24 h | Aspergillus japonicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus japonicus | isoelectrofocusing | - |
2.8 |
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Release 2023.1 (January 2023)
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