Cloned (Comment) | Organism |
---|---|
- |
Geotrichum sp. |
expressed in Escherichia coli | Geotrichum sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
SDS-PAGE | Geotrichum sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligoxyloglucan | Geotrichum sp. | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geotrichum sp. | Q8J0D2 | - |
- |
Geotrichum sp. | Q8J0D2 | M128 | - |
Purification (Comment) | Organism |
---|---|
- |
Geotrichum sp. |
Renatured (Comment) | Organism |
---|---|
recombinant protein is expressed as insoluble inclusion bodies. Purified inclusion bodies were solubilized in 8 M urea. Renaturing by dialysis results in enzymatically active enzyme | Geotrichum sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.6 | - |
- |
Geotrichum sp. |
15.31 | - |
- |
Geotrichum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heptasaccharide XXXG | - |
Geotrichum sp. | trisaccharide XG + tetrasaccharide XX | - |
? | |
additional information | enzyme has endoglucanase activity, releases two glucosyl residue segments from the main chain | Geotrichum sp. | ? | - |
? | |
oligoxyloglucan | - |
Geotrichum sp. | ? | - |
? | |
oligoxyloglucan | enzyme recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain | Geotrichum sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OXG-RCBH | - |
Geotrichum sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Geotrichum sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10% loss of activity after 10 min at pH 4 | Geotrichum sp. |
50 | - |
90% activity after 10 min at pH 4 | Geotrichum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5 | - |
Geotrichum sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | stable at 45°C | Geotrichum sp. |
4 | 8 | 90% activity at 45°C | Geotrichum sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geotrichum sp. | - |
- |
6 |
Geotrichum sp. | - |
- |
6.1 |