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Literature summary for 3.2.1.15 extracted from

  • Foda, M.S.; Rizk, I.R.S.; Gibriel, A.Y.; Basha, S.I.
    Biochemical properties of polygalacturonase, produced by Aspergillus aculeatus and Mucor pusillus (1984), Zentralbl. Mikrobiol., 139, 463-469.
No PubMed abstract available

Application

Application Comment Organism
nutrition application in extraction of juice from certain fruits and vegetables Rhizomucor pusillus
nutrition application in extraction of juice from certain fruits and vegetables Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
DSM 63261
-
Rhizomucor pusillus
-
QM 346
-
Rhizomucor pusillus QM 346
-
QM 346
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O highly esterified Rhizomucor pusillus ?
-
?
pectin + H2O highly esterified Aspergillus aculeatus ?
-
?
pectin + H2O highly esterified Rhizomucor pusillus QM 346 ?
-
?
polygalacturonate + H2O
-
Rhizomucor pusillus oligogalacturonates
-
?
polygalacturonate + H2O
-
Aspergillus aculeatus oligogalacturonates
-
?
polygalacturonate + H2O
-
Rhizomucor pusillus QM 346 oligogalacturonates
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Rhizomucor pusillus
60
-
-
Aspergillus aculeatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 60% of maximal activity, 80°C: about 40% of maximal activity Aspergillus aculeatus
30 80 30°C: about 70% of maximal activity, 80°C: about 50% of maximal activity Rhizomucor pusillus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
5 min, complete inactivation Rhizomucor pusillus
100
-
5 min, complete inactivation Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4.5
-
Aspergillus aculeatus
4.5
-
-
Rhizomucor pusillus

pH Range

pH Minimum pH Maximum Comment Organism
2 6 pH 2.0: about 20% of maximal activity, pH 6.0: about 70% of maximal activity Rhizomucor pusillus
2 6 pH 2.0: about 30% of maximal activity, pH 6.0: about 40% of maximal activity Aspergillus aculeatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1
-
notable loss of activity irrespective of the period of exposure Rhizomucor pusillus
1 8 stable for several hours, marked loss of activity after 6 days Aspergillus aculeatus