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Literature summary for 3.2.1.147 extracted from

  • Liebminger, E.; Grass, J.; Jez, J.; Neumann, L.; Altmann, F.; Strasser, R.
    Myrosinases TGG1 and TGG2 from Arabidopsis thaliana contain exclusively oligomannosidic N-glycans (2012), Phytochemistry, 84, 24-30.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P37702
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Arabidopsis thaliana Q9C5C2
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Arabidopsis thaliana Columbia-0 P37702
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Arabidopsis thaliana Columbia-0 Q9C5C2
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein all four potential N-glycosylation sites are subjected to glycosylation and are occupied by oligomannosidic structures with Man5GlcNAc2 as the main oligosaccharide, found on the majority of TGG1 glycopeptides. In addition, Man6GlcNAc2, Man7GlcNAc2 and Man8GlcNAc2 are also present on several glycosylation sites, while on Asn108 the predominant peak corresponds to Man9Glc-NAc2. TGG2 N-glycans are not altered in the mvp1 mutant Arabidopsis thaliana
glycoprotein all nine potential N-glycosylation sites are subjected to glycosylation and are occupied by oligomannosidic structures with Man5GlcNAc2 as the main oligosaccharide, found on the majority of TGG1 glycopeptides. In addition, Man6GlcNAc2, Man7GlcNAc2 and Man8GlcNAc2 are also present on several glycosylation sites, while on Asn108 the predominant peak corresponds to Man9Glc-NAc2. TGG1 from mns mutants displays incompletely processed oligomannosidic N-glycans, while TGG1 N-glycans are not altered in the mvp1 mutant Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
guard cell TGG1 is highly abundant in guard cells of leaves Arabidopsis thaliana
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leaf TGG1 is cell type-specific expressed in specialized myrosin cells Arabidopsis thaliana
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additional information TGG1 accumulates abnormally in mns mutants Arabidopsis thaliana
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additional information TGG2 accumulates abnormally in mns mutants Arabidopsis thaliana
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rosette leaf
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Arabidopsis thaliana
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Synonyms

Synonyms Comment Organism
myrosinase
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Arabidopsis thaliana
TGG1
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Arabidopsis thaliana
TGG2
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Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana TGG1 transcript levels are higher in leaves of mns1 mns2 mns3 plants up

General Information

General Information Comment Organism
additional information the enzyme is sensitive to endoglycosidase digestion Arabidopsis thaliana