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Literature summary for 3.2.1.147 extracted from
- Purification and characterization of broccoli (Brassica oleracea var. italica) myrosinase (beta-thioglucosidase glucohydrolase) (2014), J. Agric. Food Chem., 62, 11666-11671.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sinigrin | substrate inhibition | Brassica oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.086 | - |
sinigrin | Michaelis-Menten modelling, pH 7.0, 40°C | Brassica oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 157000 | - |
gel filtration | Brassica oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
broccoli, var. italica, cv. Avenger | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Brassica oleracea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1318fold from broccoli by ammonium sulfate fractionation followed by concanavalin A affinity chromatography, with an intermediate dialysis step | Brassica oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
purified enzyme, pH 7.0, 40°C | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sinigrin + H2O | - |
Brassica oleracea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 50000-55000, SDS-PAGE | Brassica oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-thioglucosidase glucohydrolase | - |
Brassica oleracea |
| myrosinase | - |
Brassica oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Brassica oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
below | Brassica oleracea |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.368 | - |
sinigrin | sinigrin substrate inhibition, Michaelis-Menten modelling, pH 7.0, 40°C | Brassica oleracea | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | Brassicaceae myrosinases are glycoproteins that have multiple forms with different molecular masses, number of subunits, subunit size and carbohydrate content | Brassica oleracea |
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Release 2023.1 (January 2023)
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