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Literature summary for 3.2.1.147 extracted from

  • Braschi, I.; Leoni, O.; Cinti, S.; Palmieri, S.; Gessa, C.
    Activity of myrosinase from Sinapis alba seeds immobilized into Ca-polygalacturonate as a simplified model of soil-root interface mucigel (2011), Plant Soil, 339, 209-218.
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General Stability

General Stability Organism
the high enzyme immobilization yield into Ca-polygalacturonate and its activity preservation under different conditions suggest that the enzyme released by plants at root level can be entrapped in root mucigel in order to preserve its activity. Enzyme activity preservation in the gel is high, being 95% of the initial activity after two months Sinapis alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.96
-
 sinigrin free enzyme, at pH 6.5 and 37°C Sinapis alba
6.28
-
 sinigrin enzyme immobilized into Ca-polygalacturonate, at pH 6.5 and 37°C Sinapis alba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 x * 140000, SDS-PAGE Sinapis alba

Organism

Organism UniProt Comment Textmining
Sinapis alba
-
 white mustard
-

Purification (Commentary)

Purification (Comment) Organism
Con-A-Sepharose column chromatography, SP-Sepharose column chromatography, and Superdex 200 gel filtration Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Sinapis alba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epi-progoitrin + H2O i.e. 2(S)-2-hydroxy-3-butenyl glucosinolate Sinapis alba ?
-
 ?
nasturtin + H2O best substrate, i.e. 2-phenylethyl glucosinolate Sinapis alba ?
-
 ?
sinalbin + H2O i.e. 4-hydroxybenzyl glucosinolate Sinapis alba ?
-
 ?
sinigrin + H2O i.e. 2-propenyl glucosinolate Sinapis alba D-glucose + isothiocyanate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 140000, SDS-PAGE Sinapis alba

Synonyms

Synonyms Comment Organism
beta-thioglucosidase
-
 Sinapis alba
MYR
-
 Sinapis alba
myrosinase
-
 Sinapis alba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 the activity of free and immobilized enzyme forms increases with temperature up to 50-60°C. Beyond this value range it decreases to about 75% of the maximal activity at 70°C Sinapis alba

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 maximal activity is maintained in a large pH interval ranging from 5.5 to 8.5. At lower pH values, free and immobilized myrosinase forms show a strong activity loss, at pH 5.0 it is about 50% of the maximal activity, whereas it is almost absent at pH 4.5 Sinapis alba