Any feedback?
Literature summary for 3.2.1.147 extracted from
- Studies on the mechanism of myrosinase. Investigation of the effect of glycosyl acceptors on enzymatic activity (1995), J. Biol. Chem., 270, 20530-20535.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | activates | Sinapis alba |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| delta-gluconolactone | poor noncompetitive inhibitor | Sinapis alba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
sinigrin | with H2O or 6.25 M methanol as solvent | Sinapis alba | |
| 0.54 | - |
sinigrin | with acetonitrile as solvent | Sinapis alba | |
| 0.57 | - |
sinigrin | with 6.25 M ethanol as solvent | Sinapis alba | |
| 0.57 | - |
sinigrin | with 12.5 M methanol as solvent | Sinapis alba | |
| 0.87 | - |
sinigrin | with dioxane as solvent | Sinapis alba | |
| 4 | - |
p-nitrophenyl-beta-D-glucopyranoside | with dioxane as solvent | Sinapis alba | |
| 12 | - |
p-nitrophenyl-beta-D-glucopyranoside | with 12.5 M methanol as solvent | Sinapis alba | |
| 17 | - |
p-nitrophenyl-beta-D-glucopyranoside | with acetonitrile as solvent | Sinapis alba | |
| 30 | - |
p-nitrophenyl-beta-D-glucopyranoside | with 6.25 M methanol as solvent | Sinapis alba | |
| 49 | - |
p-nitrophenyl-beta-D-glucopyranoside | with 0.2 M methanol as solvent | Sinapis alba | |
| 51 | - |
p-nitrophenyl-beta-D-glucopyranoside | with 6.25 M ethanol as solvent | Sinapis alba | |
| 61 | - |
p-nitrophenyl-beta-D-glucopyranoside | with water as solvent | Sinapis alba | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinapis alba | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Sinapis alba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme does not catalyze a transglycosylation reaction either with alcohols or with other suitable glycosyl acceptors | Sinapis alba | ? | - |
? | |
| p-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sinapis alba | ? | - |
? | |
| sinigrin + H2O | - |
Sinapis alba | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
