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Literature summary for 3.2.1.147 extracted from

  • Jwanny, E.W.; El-Sayed, S.T.; Rashad, M.M.; Mahmoud, A.E.; Abdallah, N.M.
    Myrosinase from roots of Raphanus sativus (1995), Phytochemistry, 39, 1301-1303.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, slight activation Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM AgNO3, 79% inhibition Raphanus sativus
ascorbic acid
-
 Raphanus sativus
Ca2+ 1 mM CaSO4, 55% inhibition Raphanus sativus
Co2+ 1 mM CoNO3, 66% inhibition Raphanus sativus
Cu2+ 1 mM CuSO4, 30% inhibition Raphanus sativus
EDTA
-
 Raphanus sativus
Fe2+ 1 mM FeSO4, 61% inhibition Raphanus sativus
K2SO4 1 mM, complete inhibition Raphanus sativus
L-Cys
-
 Raphanus sativus
Mg2+ 1 mM MgSO4, 93% inhibition Raphanus sativus
Pb2+ 1 mM Pb-acetate, 60% inhibition Raphanus sativus
PCMB
-
 Raphanus sativus
Zn2+ 1 mM ZnSO4, 37% inhibition Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 sinigrin
-
 Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
 i.e. radish
-

Purification (Commentary)

Purification (Comment) Organism
2 isoenzymes Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Raphanus sativus
-
root
-
 Raphanus sativus
-
seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucosinalbin + H2O
-
 Raphanus sativus ?
-
 ?
progoitrin + H2O
-
 Raphanus sativus additional information
-
 ?
sinigrin + H2O
-
 Raphanus sativus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Raphanus sativus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 90 min, stable Raphanus sativus
40
-
 45 min, 18% loss of activity Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 at 37°C Raphanus sativus

pH Range

pH Minimum pH Maximum Comment Organism
5.2 6.5 pH 5.2: about 50% of maximal activity, pH 6.0-6.5: optimum Raphanus sativus