Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | - |
Lepidium sativum | |
ascorbic acid | - |
Brassica juncea | |
ascorbic acid | activates | Brassica napus | |
ascorbic acid | activates | Raphanus sativus | |
ascorbic acid | activates | Sinapis alba | |
ascorbic acid | activates | Eutrema japonicum | |
ascorbic acid | maximal activation at 0.7 mM | Sinapis alba | |
epithiospecifier protein | ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. | Brassica oleracea | |
epithiospecifier protein | ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. | Aspergillus sydowii | |
epithiospecifier protein | ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. | Brassica rapa | |
epithiospecifier protein | ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. | Sinapis alba | |
epithiospecifier protein | ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. | Crambe hispanica subsp. abyssinica | |
epithiospecifier protein | ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. | Armoracia rusticana | |
epithiospecifier protein | interacts with myrosinase to promote sulfur transfer from the S-glucose moiety to the terminal alkenyl moiety. Degradation of progoitrin produces mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein | Crambe hispanica subsp. abyssinica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Armoracia rusticana | - |
- |
membrane | associated with the cytoplasmic side of internal membranes | Armoracia rusticana | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabis alpina | - |
- |
- |
Armoracia rusticana | - |
- |
- |
Aspergillus niger | - |
- |
- |
Aspergillus sydowii | - |
- |
- |
Brassica juncea | - |
- |
- |
Brassica napus | - |
i.e. oilseed rape | - |
Brassica oleracea | - |
var. bullata subvar. gemmifera, i.e. Brussel sprouts | - |
Brassica rapa | - |
var. chinensis | - |
Brassica rapa subsp. chinensis | - |
- |
- |
Brevicoryne brassicae | - |
- |
- |
Crambe hispanica subsp. abyssinica | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Erysimum cheiri | - |
- |
- |
Eutrema japonicum | - |
- |
- |
Iberis umbellata | - |
- |
- |
Lepidium sativum | - |
- |
- |
Lipaphis erysimi | - |
- |
- |
Paracolobactrum aerogenoides | - |
- |
- |
Raphanus sativus | - |
- |
- |
Sinapis alba | - |
- |
- |
Tropaeolum majus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Brassica napus | - |
cotyledon | - |
Sinapis alba | - |
leaf | - |
Raphanus sativus | - |
leaf | - |
Brassica rapa | - |
leaf | - |
Brassica rapa subsp. chinensis | - |
leaf | activity is 4-5 times higher in the outer leaves than in the stalk, the inner leaves and the centre of the Brussels sprouts | Brassica oleracea | - |
myrosin cell | - |
Brassica napus | - |
myrosin cell | - |
Raphanus sativus | - |
myrosin cell | - |
Sinapis alba | - |
seed | - |
Brassica napus | - |
seed | - |
Raphanus sativus | - |
seed | - |
Lepidium sativum | - |
seed | - |
Brassica rapa | - |
seed | - |
Sinapis alba | - |
seed | - |
Brassica juncea | - |
seed | - |
Crambe hispanica subsp. abyssinica | - |
seedling | - |
Raphanus sativus | - |
seedling | - |
Lepidium sativum | - |
seedling | root | Sinapis alba | - |
seedling | cotyledon | Brassica napus | - |
seedling | cotyledon | Sinapis alba | - |
seedling | myrosinase gene MYR2 is transcribed in all organs of the seedling, myrosinase gene Myr1 shows no transcription in seedling | Sinapis alba | - |
seedling | leaf | Sinapis alba | - |
seedling | hypocotyl | Sinapis alba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
progoitrin + H2O | - |
Brassica napus | additional information | - |
? | |
progoitrin + H2O | - |
Raphanus sativus | additional information | - |
? | |
progoitrin + H2O | - |
Sinapis alba | additional information | - |
? | |
progoitrin + H2O | - |
Brevicoryne brassicae | additional information | - |
? | |
progoitrin + H2O | - |
Crambe hispanica subsp. abyssinica | additional information | mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein | ? | |
sinigrin + H2O | - |
Aspergillus niger | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Enterobacter cloacae | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Brassica napus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Raphanus sativus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Brassica oleracea | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Aspergillus sydowii | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Lepidium sativum | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Brassica rapa | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Sinapis alba | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Tropaeolum majus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Brassica juncea | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Paracolobactrum aerogenoides | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Crambe hispanica subsp. abyssinica | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Brassica rapa subsp. chinensis | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Armoracia rusticana | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Eutrema japonicum | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Brevicoryne brassicae | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Lipaphis erysimi | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Erysimum cheiri | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Arabis alpina | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
sinigrin + H2O | - |
Iberis umbellata | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Brassica napus |
60 | - |
- |
Sinapis alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Sinapis alba |