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Literature summary for 3.2.1.147 extracted from

  • Bones, A.M.; Rossiter, J.T.
    The myrosinase-glucosinolate system, its organisation and biochemistry (1996), Physiol. Plant., 97, 194-208.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid
-
Lepidium sativum
ascorbic acid
-
Brassica juncea
ascorbic acid activates Brassica napus
ascorbic acid activates Raphanus sativus
ascorbic acid activates Sinapis alba
ascorbic acid activates Eutrema japonicum
ascorbic acid maximal activation at 0.7 mM Sinapis alba
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica oleracea
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Aspergillus sydowii
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica rapa
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Sinapis alba
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Crambe hispanica subsp. abyssinica
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Armoracia rusticana
epithiospecifier protein interacts with myrosinase to promote sulfur transfer from the S-glucose moiety to the terminal alkenyl moiety. Degradation of progoitrin produces mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein Crambe hispanica subsp. abyssinica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Armoracia rusticana
-
-
membrane associated with the cytoplasmic side of internal membranes Armoracia rusticana 16020
-

Organism

Organism UniProt Comment Textmining
Arabis alpina
-
-
-
Armoracia rusticana
-
-
-
Aspergillus niger
-
-
-
Aspergillus sydowii
-
-
-
Brassica juncea
-
-
-
Brassica napus
-
i.e. oilseed rape
-
Brassica oleracea
-
var. bullata subvar. gemmifera, i.e. Brussel sprouts
-
Brassica rapa
-
var. chinensis
-
Brassica rapa subsp. chinensis
-
-
-
Brevicoryne brassicae
-
-
-
Crambe hispanica subsp. abyssinica
-
-
-
Enterobacter cloacae
-
-
-
Erysimum cheiri
-
-
-
Eutrema japonicum
-
-
-
Iberis umbellata
-
-
-
Lepidium sativum
-
-
-
Lipaphis erysimi
-
-
-
Paracolobactrum aerogenoides
-
-
-
Raphanus sativus
-
-
-
Sinapis alba
-
-
-
Tropaeolum majus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Brassica napus
-
cotyledon
-
Sinapis alba
-
leaf
-
Raphanus sativus
-
leaf
-
Brassica rapa
-
leaf
-
Brassica rapa subsp. chinensis
-
leaf activity is 4-5 times higher in the outer leaves than in the stalk, the inner leaves and the centre of the Brussels sprouts Brassica oleracea
-
myrosin cell
-
Brassica napus
-
myrosin cell
-
Raphanus sativus
-
myrosin cell
-
Sinapis alba
-
seed
-
Brassica napus
-
seed
-
Raphanus sativus
-
seed
-
Lepidium sativum
-
seed
-
Brassica rapa
-
seed
-
Sinapis alba
-
seed
-
Brassica juncea
-
seed
-
Crambe hispanica subsp. abyssinica
-
seedling
-
Raphanus sativus
-
seedling
-
Lepidium sativum
-
seedling root Sinapis alba
-
seedling cotyledon Brassica napus
-
seedling cotyledon Sinapis alba
-
seedling myrosinase gene MYR2 is transcribed in all organs of the seedling, myrosinase gene Myr1 shows no transcription in seedling Sinapis alba
-
seedling leaf Sinapis alba
-
seedling hypocotyl Sinapis alba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
progoitrin + H2O
-
Brassica napus additional information
-
?
progoitrin + H2O
-
Raphanus sativus additional information
-
?
progoitrin + H2O
-
Sinapis alba additional information
-
?
progoitrin + H2O
-
Brevicoryne brassicae additional information
-
?
progoitrin + H2O
-
Crambe hispanica subsp. abyssinica additional information mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein ?
sinigrin + H2O
-
Aspergillus niger D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Enterobacter cloacae D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brassica napus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Raphanus sativus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brassica oleracea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Aspergillus sydowii D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Lepidium sativum D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Tropaeolum majus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brassica juncea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Paracolobactrum aerogenoides D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Crambe hispanica subsp. abyssinica D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brassica rapa subsp. chinensis D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Armoracia rusticana D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Eutrema japonicum D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brevicoryne brassicae D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Lipaphis erysimi D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Erysimum cheiri D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Arabis alpina D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Iberis umbellata D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Brassica napus
60
-
-
Sinapis alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Sinapis alba