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Literature summary for 3.2.1.147 extracted from

  • Ohtsuru, M.; Hata, T.
    Molecular properties of multiple forms of plant myrosinase (1972), Agric. Biol. Chem., 36, 2495-2503.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, enzyme consists of at least 4 subunits, myrosinase F-IIB, SDS-PAGE Sinapis sp.
40000
-
x * 40000, enzyme consists of at least 4 subunits, myrosinase F-IA, F-IB and F-IIA, SDS-PAGE Sinapis sp.
124000
-
myrosinase F-IIB, equilibrium sedimentation Sinapis sp.
125000
-
myrosinase F-IIB, gel filtration Sinapis sp.
150000
-
myrosinase F-IB, gel filtration Sinapis sp.
152000
-
myrosinase F-IB, equilibrium sedimentation Sinapis sp.

Organism

Organism UniProt Comment Textmining
Sinapis sp.
-
myrosinase F-IA, D-IB, F-IIA and F-IIB
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein hexose content of enzyme F-IA is 15.8%, hexose content of enzyme F-IB is 17.8%, hexose content of enzyme F-IIA is 22.5%, hexose content of enzyme F-IIB is 8.6% Sinapis sp.

Purification (Commentary)

Purification (Comment) Organism
-
Sinapis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Sinapis sp. D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?

Subunits

Subunits Comment Organism
? x * 30000, enzyme consists of at least 4 subunits, myrosinase F-IIB, SDS-PAGE Sinapis sp.
? x * 40000, enzyme consists of at least 4 subunits, myrosinase F-IA, F-IB and F-IIA, SDS-PAGE Sinapis sp.