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Literature summary for 3.2.1.147 extracted from

  • Ohtsuru, M.; Hata, T.
    General characterization of the intracellular myrosinase from Aspergillus niger (1973), Agric. Biol. Chem., 37, 2543-2548.
No PubMed abstract available
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Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 26% inhibition Aspergillus niger
amygdalin 0.02 M, 14% inhibition Aspergillus niger
arbutin 0.1 M, 21% inhibition Aspergillus niger
delta-gluconolactone 1 mM, 62% inhibition Aspergillus niger
diisopropyl fluorophosphate 1 mM, 18% inhibition Aspergillus niger
EDTA 1 mM, 20% inhibition Aspergillus niger
Fe3+ 1 mM FeCl3, 26% inhibition Aspergillus niger
HgCl2 1 mM HgCl2, 95% inhibition Aspergillus niger
Mg2+ 1 mM MgCl2, 23% inhibition Aspergillus niger
NaBr 1 mM, 16% inhibition Aspergillus niger
PCMB 0.06 mM, 91% inhibition Aspergillus niger
Salicin 0.1 M, 15% inhibition Aspergillus niger
Sn2+ 1 mM, SnCl2, 66% inhibition Aspergillus niger
Zn2+ 1 mM ZnCl2, 28% inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 4-nitrophenyl beta-D-glucopyranoside
-
 Aspergillus niger
3.3
-
 sinigrin
-
 Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Aspergillus niger 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, stimulates Aspergillus niger
Cu+ 1 mM, stimulates Aspergillus niger
Cu2+ 1 mM, stimulates Aspergillus niger
Mn2+ 1 mM, stimulates Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 AKU 3302
-
Aspergillus niger AKU 3302
-
 AKU 3302
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.58
-
 hydrolysis of p-nitrophenyl beta-glucoside Aspergillus niger
1.916
-
 hydrolysis of sinigrin Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger AKU 3302 4-nitrophenol + beta-D-glucopyranose
-
 ?
sinigrin + H2O
-
 Aspergillus niger D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Aspergillus niger AKU 3302 D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.8, 20 min, stable Aspergillus niger
35
-
 pH 7.8, 20 min, about 40% loss of activity Aspergillus niger
40
-
 pH 7.8, 20 min, complete inactivation Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 about 35% of maximal activity at pH 5.0 and at pH 7.5 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 5°C, 24 h, about 20% loss of activity Aspergillus niger
7.6 8 5°C, 24 h, stable Aspergillus niger
9
-
 5°C, 24 h, about 55% loss of activity Aspergillus niger