Literature summary for 3.2.1.143 extracted from

Tavassoli, M.; Tavassoli, M.H.; Shall, S.
Isolation and purification of poly(ADP-ribose) glycohydrolase from pig thymus (1983), Eur. J. Biochem., 135, 449-455.

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	10 mM, stimulates by 70%	Sus scrofa
dithiothreitol	10 mM, stimulates by 70%	Sus scrofa

Inhibitors	Comment	Organism
1-Naphthylamine	1 mM, 45% inhibition	Sus scrofa
2-naphthalene sulfonic acid	5 mM, 26% inhibition	Sus scrofa
2-Naphthylamine	1 mM, 50% inhibition	Sus scrofa
4-amino-3-hydroxynaphthalene 1-sulfonic acid	5 m, 79% inhibition	Sus scrofa
8-bromoadenosine 3',5'-monophosphate	5 mM, 13% inhibition	Sus scrofa
8-methylaminoadenosine 3',5'-monophosphate	5 mM, 14% inhibition	Sus scrofa
adenine(5'-9)ribofuranoside	5 mM, 24% inhibition	Sus scrofa
adenosine 5'-carboxylic acid	5 mM, 26% inhibition	Sus scrofa
ADP-ribose	-	Sus scrofa
CaCl2	5 mM 86% inhibition	Sus scrofa
cAMP	-	Sus scrofa
cyclic dAMP	5 mM, 34% inhibition	Sus scrofa
DNA	single-stranded DNA, no inhibition by double-stranded DNA	Sus scrofa
histone	histone H1, H2A, H2B and H3	Sus scrofa
MgCl2	10 mM, 18% inhibition	Sus scrofa
N1-naphthylethanenediamine	5 mM, 70% inhibition	Sus scrofa
NaCl	400 mM, 75% inhibition	Sus scrofa
naphthylamine	-	Sus scrofa
Poly(A)	-	Sus scrofa
poly(I)	-	Sus scrofa
poly(L-Arg)	-	Sus scrofa
poly(L-Lys)	-	Sus scrofa
poly(L-Orn)	-	Sus scrofa
polyvinylsulfate	-	Sus scrofa
protamine	-	Sus scrofa

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0018	-	Poly(ADP-ribose)	as monomer units	Sus scrofa

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
59000	-	gel filtration	Sus scrofa

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Comment)	Organism
-	Sus scrofa

Source Tissue	Comment	Organism	Textmining
thymus	-	Sus scrofa	-

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
13.8	-	-	Sus scrofa

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly(ADP-ribose)n + H2O	-	Sus scrofa	ADP-ribose	-	?

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Sus scrofa

pH Minimum	pH Maximum	Comment	Organism
6	9	the enzyme is active between pH 6 and 9	Sus scrofa

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1	-	2-Naphthylamine	-	Sus scrofa