Cloned (Comment) | Organism |
---|---|
gene treZ, codon-optimized synthetic gene, recombinant expression of SUMO-fused enzyme in Escherichi coli strains BL21 (DE3) and Origami (DE3), subcloning in Escherichia coli strain JM109, host strain and induction strategy optimization for high-efficiency expression, method reaching a maximum of 204.6 U/ml for the soluble enzyme, detailed overview | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sulfolobus acidocaldarius | maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose | ? | - |
? | |
additional information | Sulfolobus acidocaldarius MT4 | maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q53641 | - |
- |
Sulfolobus acidocaldarius MT4 | Q53641 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose | Sulfolobus acidocaldarius MT4 | ? | - |
? | |
additional information | maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain | Sulfolobus acidocaldarius MT4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Maltooligosyl trehalose trehalohydrolase | - |
Sulfolobus acidocaldarius |
MTHase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
recombinant enzyme | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | recombinant enzyme | Sulfolobus acidocaldarius |