Any feedback?
Literature summary for 3.2.1.141 extracted from
- Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis (2007), J. Microbiol. Biotechnol., 17, 123-129.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| IPTG | transcription induced by 1 mM | Metallosphaera hakonensis |  |
| additional information | addition of 4-alpha-glucanotransferase increases the yield of trehalose production from maltooligosaccharide substrates supplying longer maltooligosaccharides that may be better substrates for the sequential reaction catalyzed by MTS and MTH | Metallosphaera hakonensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS | Metallosphaera hakonensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63700 | - |
sequence analysis | Metallosphaera hakonensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metallosphaera hakonensis | - |
JCM 8857 | - |
| Metallosphaera hakonensis JCM 8857 | - |
JCM 8857 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+-NTA-agarose affinity chromatography | Metallosphaera hakonensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase | Metallosphaera hakonensis | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
| 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase | Metallosphaera hakonensis JCM 8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
| maltotriosyltrehalose + H2O | - |
Metallosphaera hakonensis | maltotriose + alpha,alpha'-trehalose | - |
? | |
| maltotriosyltrehalose + H2O | - |
Metallosphaera hakonensis JCM 8857 | maltotriose + alpha,alpha'-trehalose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Maltooligosyl trehalose trehalohydrolase | - |
Metallosphaera hakonensis |
| MTH | - |
Metallosphaera hakonensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Metallosphaera hakonensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
retainis 80% of the activity for 48 h, activity is gradually abolished at above 85°C | Metallosphaera hakonensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Metallosphaera hakonensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
