Any feedback?
Literature summary for 3.2.1.141 extracted from
- Expression, purification, and characterization of the maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092 (2006), J. Agric. Food Chem., 54, 7105-7112.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | EDTA does not activate. Expression levels of both wild-type and His-tagged MTHases are higher in the absence of IPTG than in the presence of 0.5 mM IPTG induction | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pET-15b-deltaH-treZ vector expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits at 4 mM | Saccharolobus solfataricus |  |
| Hg2+ | 0.4 and 4 mM strongly inhibit | Saccharolobus solfataricus | |
| additional information | EDTA, Na+, K+, Mg2+, Ca2+, and Zn2+ do not inhibit | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.63 | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
| 5.63 | - |
maltohexaose | - |
Saccharolobus solfataricus | |
| 5.66 | - |
maltotetraosyl trehalose | - |
Saccharolobus solfataricus | |
| 5.86 | - |
maltopentaose | - |
Saccharolobus solfataricus | |
| 5.89 | - |
maltopentaosyl trehalose | - |
Saccharolobus solfataricus | |
| 7.22 | - |
maltotriosyl trehalose | - |
Saccharolobus solfataricus | |
| 10.9 | - |
maltotetraose | - |
Saccharolobus solfataricus | |
| 11.1 | - |
maltosyl trehalose | - |
Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Na+, K+, Mg2+, Ca2+, and Zn2+ do not activate | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
| 64240 | - |
2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
| 66000 | - |
1 * 66000, SDS-PAGE, His-tagged enzyme | Saccharolobus solfataricus |
| 68700 | - |
gel filtration, His-tagged enzyme | Saccharolobus solfataricus |
| 128800 | - |
gel filtration, wild-type enzyme | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | P95867 | ATCC 35092 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type enzyme purified by heat treatment, nucleic acid precipitation, and ion-exchange chromatography, 12fold. His-tagged MTHase partially purified from the cell-free extract directly by metal chelating chromatography, 14.7fold | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | 12 | wild-type, 12fold purified | Saccharolobus solfataricus |
| 21.1 | - |
His-tagged enzyme, crude extract | Saccharolobus solfataricus |
| 67.5 | - |
wild-type, crude extract | Saccharolobus solfataricus |
| 310 | - |
His-tagged enzyme, 14.7fold purified | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltoheptaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
| maltohexaose + H2O | for glucose formation, maltohexaose exhibits the highest formation rate | Saccharolobus solfataricus | ? | - |
? | |
| maltopentaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
| maltopentaosyltrehalose + H2O | trehalose formation rate increases as the degree of polymerization value of the substrate increases. maltopentaosyl trehalose is the most preferred substrate for trehalose formation | Saccharolobus solfataricus | maltopentaose + alpha,alpha'-trehalose | - |
? | |
| maltosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltose + alpha,alpha'-trehalose | - |
? | |
| maltotetraose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
| maltotetraosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltotetraose + alpha,alpha'-trehalose | - |
? | |
| maltotriosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltotriose + alpha,alpha'-trehalose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
| monomer | 1 * 66000, SDS-PAGE, His-tagged enzyme | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Maltooligosyl trehalose trehalohydrolase | - |
Saccharolobus solfataricus |
| MTHase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 85 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
retains all of its activity for 2 h | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
| 5.49 | - |
maltotetraose | - |
Saccharolobus solfataricus | |
| 6.01 | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
| 17.7 | - |
maltopentaose | - |
Saccharolobus solfataricus | |
| 18.2 | - |
maltohexaose | - |
Saccharolobus solfataricus | |
| 193 | - |
maltosyl trehalose | - |
Saccharolobus solfataricus | |
| 1030 | - |
maltotriosyl trehalose | - |
Saccharolobus solfataricus | |
| 1190 | - |
maltotetraosyl trehalose | - |
Saccharolobus solfataricus | |
| 1230 | - |
maltopentaosyl trehalose | - |
Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 11 | - |
Saccharolobus solfataricus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
