Application | Comment | Organism |
---|---|---|
molecular biology | screening instrument to find alpha-glucuronidase genes in DNA libraries in solid phase that enables higher throughput compared to liquid phase assays - screening of 50000 clones per 15-cm petri dish, addition of beta-xylosidase increases signal, the enzyme is important in facilitating the cellulose breakdown for biofuel production | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21(DE3)pLysE, Luria-Bertani broth, 37°C, plasmid pET29-Gste-AG-his-S | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O | Geobacillus stearothermophilus | hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds | D-glucuronic acid + ? | - |
? | |
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O | Geobacillus stearothermophilus T6 | hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds | D-glucuronic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q09LY5 | T6 | - |
Geobacillus stearothermophilus T6 | Q09LY5 | T6 | - |
Purification (Comment) | Organism |
---|---|
soluble phase onto HisTrap HP column, eluted with imidazole gradient in sodium phosphate buffer, pH 8, and 300 mM sodium chloride | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O | hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds | Geobacillus stearothermophilus | D-glucuronic acid + ? | - |
? | |
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O | hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds | Geobacillus stearothermophilus T6 | D-glucuronic acid + ? | - |
? | |
aldouronic acid + H2O | mixtures of aldobiouronic, aldottriouronic, aldotetraouronic, and aldopentaouronic acid, glucuronic acid groups attached via 1,2-linkage to xylose or non-reducing terminal residue of xylose-oligosaccharides | Geobacillus stearothermophilus | D-xylose + 4-O-methyl-D-glucuronic acid | direct product of aldobiouronic acid | ? | |
aldouronic acid + H2O | mixtures of aldobiouronic, aldottriouronic, aldotetraouronic, and aldopentaouronic acid, glucuronic acid groups attached via 1,2-linkage to xylose or non-reducing terminal residue of xylose-oligosaccharides | Geobacillus stearothermophilus T6 | D-xylose + 4-O-methyl-D-glucuronic acid | direct product of aldobiouronic acid | ? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucuronidase | - |
Geobacillus stearothermophilus |