Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.139 extracted from

  • Nagy, T.; Nurizzo, D.; Davies, G.J.; Biely, P.; Lakey, J.H.; Bolam, D.N.; Gilbert, H.J.
    The alpha-glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants (2003), J. Biol. Chem., 278, 20286-20292.
    View publication on PubMed
Search for more literature for 3.2.1.139

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of mutant E292A in complex with its substrate aldobiouronic acid Cellvibrio japonicus

Protein Variants

Protein Variants Comment Organism
E292A inactive mutant enzyme Cellvibrio japonicus
K288A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.003% of wild-type value Cellvibrio japonicus
K360A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.019% of wild-type value Cellvibrio japonicus
R325A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.000096% of wild-type value Cellvibrio japonicus
V210A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 38% of wild-type value Cellvibrio japonicus
V210G kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is identical to wild-type value Cellvibrio japonicus
V210N kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 9.4% of wild-type value Cellvibrio japonicus
V210S kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 4.3% of wild-type value Cellvibrio japonicus
W160A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.0064% of wild-type value Cellvibrio japonicus
W543A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.069% of wild-type value Cellvibrio japonicus

Inhibitors

Inhibitors Comment Organism Structure
4-O-Methyl-D-glucuronic acid
-
 Cellvibrio japonicus
D-glucose
-
 Cellvibrio japonicus
D-glucuronic acid
-
 Cellvibrio japonicus
D-xylose
-
 Cellvibrio japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210G Cellvibrio japonicus
0.21
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
0.69
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K288A Cellvibrio japonicus
0.82
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W543A Cellvibrio japonicus
0.86
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210A Cellvibrio japonicus
1.6
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K360A Cellvibrio japonicus
2.1
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W160A Cellvibrio japonicus

Organism

Organism UniProt Comment Textmining
Cellvibrio japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cellvibrio japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus 4-nitrophenyl-D-xylopyranoside + 4-O-methyl-alpha-D-glucuronate
-
 ?
aldobiouronic acid + H2O
-
 Cellvibrio japonicus ?
-
 ?

Synonyms

Synonyms Comment Organism
GlcA67A
-
 Cellvibrio japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K288A Cellvibrio japonicus
0.09
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W160A Cellvibrio japonicus
0.2
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K360A Cellvibrio japonicus
0.38
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W543A Cellvibrio japonicus
127
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210G Cellvibrio japonicus
143
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
220
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210A Cellvibrio japonicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.54
-
 4-O-methyl-glucuronic acid 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.84
-
 glucuronic acid 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
64.3
-
 glucuronic acid 37°C, pH 7.0, mutant enzyme V210N Cellvibrio japonicus
69.5
-
 xylose 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
77.1
-
 glucose 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
78.3
-
 glucuronic acid 37°C, pH 7.0, mutant enzyme V210S Cellvibrio japonicus