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Literature summary for 3.2.1.139 extracted from
- A thermostable non-xylanolytic alpha-glucuronidase of Thermotoga maritima MSB8 (2003), Biosci. Biotechnol. Biochem., 67, 2359-2364.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | maximal activation at 150 mM | Thermotoga maritima |  |
| DTT | activates | Thermotoga maritima | |
| L-cysteine | maximal activation at 0.6 mM, 15% of the activation with 2-mercaptoethanol | Thermotoga maritima | |
| NAD+ | maximal activation at 0.5 mM | Thermotoga maritima | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
p-nitrophenyl alpha-D-glucuronopyranoside | - |
Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | P96105 | - |
- |
| Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | P96105 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl alpha-D-glucuronopyranoside + H2O | - |
Thermotoga maritima | p-nitrophenol + D-glucuronate | - |
? | |
| p-nitrophenyl alpha-D-glucuronopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | p-nitrophenol + D-glucuronate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Agu4B | - |
Thermotoga maritima |
| non-xylanolytic alpha-glucuronidase | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | 60°C: about 50% of maximal activity, 90°C: about 80% of maximal activity | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, stable up to | Thermotoga maritima |
| 80 | - |
30 min, 10% loss of activity | Thermotoga maritima |
| 90 | - |
30 min, complete inactivation | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
p-nitrophenyl alpha-D-glucuronopyranoside | - |
Thermotoga maritima | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 12 | 30 min, stable between pH 4 and pH 12 | Thermotoga maritima |
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