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Literature summary for 3.2.1.137 extracted from

  • Takegawa, K.; Miki, S.; Jikibara, T.; Iwahara, S.
    Purification and characterization of exo-alpha-D-mannosidase from a Cellulomonas sp. (1989), Biochim. Biophys. Acta, 991, 431-437.
No PubMed abstract available
Search for more literature for 3.2.1.137

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 97% inhibition at 1 mM Cellulomonas sp.
Cu2+ 88% inhibition at 1 mM Cellulomonas sp.
EDTA complete inhibition at 1 mM Cellulomonas sp.
Hg2+ 98% inhibition at 1 mM Cellulomonas sp.
Zn2+ 68% inhibition at 1 mM Cellulomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 D-mannan pH 7, 37°C, hydrolysis of alpha-1,2-linkage in yeast mannan Cellulomonas sp.
3.2
-
 D-mannan pH 7, 37°C, hydrolysis of alpha-1,6-linkage in yeast mannan Cellulomonas sp.
200
-
 D-mannan pH 7, 37°C, hydrolysis of alpha-1,3-linkage in yeast mannan Cellulomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
450000
-
 gel filtration Cellulomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannan + H2O Cellulomonas sp. hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Cellulomonas sp.
-
 production of the enzyme strongly induced by yeast extract, 2 different MW forms
-

Purification (Commentary)

Purification (Comment) Organism
high molecular weight form Cellulomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.8
-
-
 Cellulomonas sp.

Storage Stability

Storage Stability Organism
-20°C Cellulomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannan hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose, hydrolysis rate of alpha-1,3-linkage is very low Cellulomonas sp. D-mannose
-
 ?
D-mannan + H2O hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose Cellulomonas sp. D-mannose
-
 ?
additional information only slight activity towards 4-nitrophenyl-alpha-D-mannoside and no hydrolysis of O-alpha-D-mannopyranosyl-1,2-D-mannitol Cellulomonas sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cellulomonas sp.
50
-
-
 Cellulomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, stable Cellulomonas sp.
50
-
 10 min, 50% loss of activity Cellulomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
 Cellulomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5: about 20% of activity maximum, pH 9: about 15% of activity maximum Cellulomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 4°C, 48 h, stable Cellulomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Cellulomonas sp. pH range 3.5-10 in a 0-60% sucrose gradient
-
 3.2