Literature summary for 3.2.1.136 extracted from

St. John, F.J.; Godwin, D.K.; Preston, J.F.; Pozharski, E.; Hurlbert, J.C.
Crystallization and crystallographic analysis of Bacillus subtilis xylanase C (2009), Acta Crystallogr. Sect. F, 65, 499-503.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene ynfF, expression of His-tagged XynC in Escherichia coli strain BL21(DE3)	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged XynC, 5 mg/ml protein in 20 mM HEPES, 150 mM NaCl pH 7.2, is mixed with 0.2 M sodium tartrate and 20% PEG 3350, which results in a crystal-positive condition with 0.1 M sodium malonate pH 7.0, X-ray diffraction structure determination and analysis at 1.64-2.7 A resolution, molecular replacement	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	Q45070	subsp. subtilis, strain 168, gene ynfF	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged XynC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
xylanase C	-	Bacillus subtilis
XynC	-	Bacillus subtilis

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Release 2023.1 (January 2023)