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Literature summary for 3.2.1.135 extracted from

  • Buddelmeijer, N.; Francetic, O.; Pugsley, A.P.
    Green fluorescent chimeras indicate nonpolar localization of pullulanase secreton components PulL and PulM (2006), J. Bacteriol., 188, 2928-2935.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
all pul genes expressed in Escherichia coli K-12 strains, all pull genes except pulL expressed from plasmid pCHAP1217, pulL expressed from plasmid pCHAP710. Overexpression of gfp-pulL or gfp-pulM in Escherichia coli Klebsiella oxytoca

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information pullulanase secreton (type II secretion system) components PulM and PulL do not localize specifically to the cell poles, Pul secreton is distributed over the cell surface. GFP-PulM is evenly distributed over the cell envelope, whereas GFP-PulL is barely detectable in the envelope. When produced together with all other secreton components, GFP-PulL is circumferentially distributed, with numerous brighter patches. Envelope-association of GFP-PulL is almost completely abolished when native PulL is also produced. GFP-PulM and GFP-PulL both appear in spherical polar foci when overexpressed Klebsiella oxytoca
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Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca
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Synonyms

Synonyms Comment Organism
Pul
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 Klebsiella oxytoca
pullulanase
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 Klebsiella oxytoca