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Literature summary for 3.2.1.133 extracted from
- Evidence for the improvement of thermostability of the maltogenic alpha-amylase of Aspergillus niger by negative pressure (2012), Starch, 64, 646-651.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is used for the production of maltose syrup from starch | Aspergillus niger |
General Stability
| General Stability | Organism |
|---|---|
| at atmospheric pressure the enzyme is unstable at 60 and 70°C, while under negative pressure (_-200 mbar) the enzyme shows its activity on the starch at up to 70°C | Aspergillus niger |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | calcium can stabilize the enzyme activity up to 50°C at atmospheric pressure (% of residual activities at 50, 60, and 70°C are 100, 4.93, 0, respectively) | Aspergillus niger |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Aspergillus niger | the enzyme shows higher affinity to the starch at negative pressure (-200 mbar) compared to the atmospheric pressure | maltose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gelatinized corn starch + H2O | - |
Aspergillus niger | ? | - |
? | |
| starch + H2O | the enzyme shows higher affinity to the starch at negative pressure (-200 mbar) compared to the atmospheric pressure | Aspergillus niger | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAZy-GH13 | - |
Aspergillus niger |
| maltogenic alpha-amylase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | the enzyme is stable up to 40°C at atmospheric pressure, but the activity of the maltogenic alpha-amylase is drastically reduced above 40°C. Hhigher temperatures (60 and 70°C) are destructive to the enzyme even in the presence of substrate starch | Aspergillus niger |
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Release 2023.1 (January 2023)
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