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Literature summary for 3.2.1.133 extracted from

  • Ben Mabrouk, S.; Ayadi-Zouari, D.; Ben Hlima, H.; Bejar, S.
    Changes in the catalytic properties and substrate specificity of Bacillus sp. US149 maltogenic amylase by mutagenesis of residue 46 (2013), J. Ind. Microbiol. Biotechnol., 40, 947-953.
    View publication on PubMed
Search for more literature for 3.2.1.133

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
D46G the mutant behaves similar to the wild type enzyme regarding thermoactivity, thermostability and pH profile. The Km values of the mutant for all substrates are strongly increased compared to the wild type enzyme Bacillus sp. (in: Bacteria)
D46N the mutant behaves similar to the wild type enzyme regarding kinetic parameters, thermoactivity, thermostability and pH profile Bacillus sp. (in: Bacteria)
D46V the mutant behaves similar to the wild type enzyme regarding thermoactivity, thermostability and pH profile. The affinity and catalytic efficiency of the mutant toward beta-cyclodextrin are increased 5fold as compared with the wild type enzyme Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67500
-
 x * 67500, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A7DWA8
-
-
Bacillus sp. (in: Bacteria) US149 A7DWA8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and fast-performance liquid chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyclodextrin + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
beta-cyclodextrin + H2O
-
 Bacillus sp. (in: Bacteria) US149 ?
-
 ?
potato starch + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
potato starch + H2O
-
 Bacillus sp. (in: Bacteria) US149 ?
-
 ?
pullulan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
pullulan + H2O
-
 Bacillus sp. (in: Bacteria) US149 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67500, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
MAase
-
 Bacillus sp. (in: Bacteria)
maltogenic amylase
-
 Bacillus sp. (in: Bacteria)
MAUS149
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 half-life at 55°C is about 15 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacillus sp. (in: Bacteria)