Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga neapolitana |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Thermotoga neapolitana |
Protein Variants | Comment | Organism |
---|---|---|
D135A | inactive | Thermotoga neapolitana |
D169N | inactive | Thermotoga neapolitana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by acarbose | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | B5ARZ9 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap SP column chromatography and Superdex 200 gel filtration | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucoside + H2O | poor substrate | Thermotoga neapolitana | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl maltopyranoside + H2O | excellent substrate | Thermotoga neapolitana | 4-nitrophenol + maltose | - |
? | |
amylopectin + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
amylose + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
glycogen + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
maltotriose + H2O | - |
Thermotoga neapolitana | maltose + D-glucose | - |
? | |
additional information | does not hydrolyze cyclodextrin, pullulan and acarbose | Thermotoga neapolitana | ? | - |
? | |
starch + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Thermotoga neapolitana |
Synonyms | Comment | Organism |
---|---|---|
alpha-amylase | - |
Thermotoga neapolitana |
AmyB | - |
Thermotoga neapolitana |
maltogenic amylase | - |
Thermotoga neapolitana |