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Literature summary for 3.2.1.133 extracted from

  • Jun, S.Y.; Kim, J.S.; Choi, K.H.; Cha, J.; Ha, N.C.
    Structure of a novel alpha-amylase AmyB from Thermotoga neapolitana that produces maltose from the nonreducing end of polysaccharides (2013), Acta Crystallogr. Sect. D, 69, 442-450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga neapolitana

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Thermotoga neapolitana

Protein Variants

Protein Variants Comment Organism
D135A inactive Thermotoga neapolitana
D169N inactive Thermotoga neapolitana

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by acarbose Thermotoga neapolitana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Thermotoga neapolitana

Organism

Organism UniProt Comment Textmining
Thermotoga neapolitana B5ARZ9
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap SP column chromatography and Superdex 200 gel filtration Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucoside + H2O poor substrate Thermotoga neapolitana 4-nitrophenol + D-glucose
-
?
4-nitrophenyl maltopyranoside + H2O excellent substrate Thermotoga neapolitana 4-nitrophenol + maltose
-
?
amylopectin + H2O
-
Thermotoga neapolitana maltose + ?
-
?
amylose + H2O
-
Thermotoga neapolitana maltose + ?
-
?
glycogen + H2O
-
Thermotoga neapolitana maltose + ?
-
?
maltotriose + H2O
-
Thermotoga neapolitana maltose + D-glucose
-
?
additional information does not hydrolyze cyclodextrin, pullulan and acarbose Thermotoga neapolitana ?
-
?
starch + H2O
-
Thermotoga neapolitana maltose + ?
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Thermotoga neapolitana

Synonyms

Synonyms Comment Organism
alpha-amylase
-
Thermotoga neapolitana
AmyB
-
Thermotoga neapolitana
maltogenic amylase
-
Thermotoga neapolitana