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Literature summary for 3.2.1.132 extracted from

  • Fukamizo, T.; Brzezinski, R.
    Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function (1997), Biochem. Cell Biol., 75, 687-696.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans TK24 Streptomyces sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
the substrate binding cleft is composed of six monosaccharide binding subsites Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
D37E relative activity to wild-type enzyme is 20-90% Streptomyces sp.
D37N relative activity to wild-type enzyme is 20-90% Streptomyces sp.
D40G relative activity to wild-type enzyme is 0.02-0.8% Streptomyces sp.
D40N relative activity to wild-type enzyme is 0.02-0.8% Streptomyces sp.
D6N relative activity to wild-type enzyme is 20-90% Streptomyces sp.
E22A relative activity to wild-type enzyme is 0.02-0.8% Streptomyces sp.
E22D relative activity to wild-type enzyme is 0.02-0.8% Streptomyces sp.
E22Q relative activity to wild-type enzyme is 0.02-0.8% Streptomyces sp.
E36Q relative activity to wild-type enzyme is 20-90% Streptomyces sp.
R205A relative activity to wild-type enzyme is 0.1-0.2% Streptomyces sp.
R205H relative activity to wild-type enzyme is 0.1-0.2% Streptomyces sp.
R205Y relative activity to wild-type enzyme is 0.1-0.2% Streptomyces sp.
W101F no effect to the activity in hydrolysing chitohexaose, but just 70-90% activity against 30% acetylated chitosan in contrast to the wild-type enzyme Streptomyces sp.
W227F no effect to the activity in hydrolysing chitohexaose, but just 70-90% activity against 30% acetylated chitosan in contrast to the wild-type enzyme Streptomyces sp.
W28F no effect to the activity in hydrolysing chitohexaose, but just 70-90% activity against 30% acetylated chitosan in contrast to the wild-type enzyme Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
N174
-