Application | Comment | Organism |
---|---|---|
degradation | xylan degradation by endoxylanase Xyn10A is enhanced by about 10% in presence of AguA | Pseudothermotoga thermarum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudothermotoga thermarum |
Crystallization (Comment) | Organism |
---|---|
structural analysis. Residue E288 acts at the catalytic proton donor, D367 and E395 are likely nucleophilic bases | Pseudothermotoga thermarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose | pH 6.5, 80°C | Pseudothermotoga thermarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Pseudothermotoga thermarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudothermotoga thermarum | F7YUS1 | - |
- |
Pseudothermotoga thermarum DSM 5069 | F7YUS1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57.5 | - |
pH 6.5, 80°C | Pseudothermotoga thermarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O | - |
Pseudothermotoga thermarum | 4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose | - |
ir | |
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O | - |
Pseudothermotoga thermarum DSM 5069 | 4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose | - |
ir | |
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O | - |
Pseudothermotoga thermarum | ? | - |
? | |
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O | - |
Pseudothermotoga thermarum DSM 5069 | ? | - |
? | |
aldobiuronic acid + H2O | - |
Pseudothermotoga thermarum | D-xylose + ? | - |
? | |
aldobiuronic acid + H2O | - |
Pseudothermotoga thermarum DSM 5069 | D-xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Pseudothermotoga thermarum |
Synonyms | Comment | Organism |
---|---|---|
AguA | - |
Pseudothermotoga thermarum |
Theth_0154 | - |
Pseudothermotoga thermarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pseudothermotoga thermarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
2 h, 98% residual activity | Pseudothermotoga thermarum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
117 | - |
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose | pH 6.5, 80°C | Pseudothermotoga thermarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pseudothermotoga thermarum |