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Literature summary for 3.2.1.131 extracted from

  • Wang, L.; Shi, H.; Xu, B.; Li, X.; Zhang, Y.; Wang, F.
    Characterization of Thermotoga thermarum DSM 5069 alpha-glucuronidase and synergistic degradation of xylan (2016), BioResources, 11, 5767-5779.
No PubMed abstract available

Application

Application Comment Organism
degradation xylan degradation by endoxylanase Xyn10A is enhanced by about 10% in presence of AguA Pseudothermotoga thermarum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudothermotoga thermarum

Crystallization (Commentary)

Crystallization (Comment) Organism
structural analysis. Residue E288 acts at the catalytic proton donor, D367 and E395 are likely nucleophilic bases Pseudothermotoga thermarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose pH 6.5, 80°C Pseudothermotoga thermarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Pseudothermotoga thermarum

Organism

Organism UniProt Comment Textmining
Pseudothermotoga thermarum F7YUS1
-
-
Pseudothermotoga thermarum DSM 5069 F7YUS1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57.5
-
pH 6.5, 80°C Pseudothermotoga thermarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
-
Pseudothermotoga thermarum 4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
-
ir
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
-
Pseudothermotoga thermarum DSM 5069 4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
-
ir
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
-
Pseudothermotoga thermarum ?
-
?
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
-
Pseudothermotoga thermarum DSM 5069 ?
-
?
aldobiuronic acid + H2O
-
Pseudothermotoga thermarum D-xylose + ?
-
?
aldobiuronic acid + H2O
-
Pseudothermotoga thermarum DSM 5069 D-xylose + ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Pseudothermotoga thermarum

Synonyms

Synonyms Comment Organism
AguA
-
Pseudothermotoga thermarum
Theth_0154
-
Pseudothermotoga thermarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Pseudothermotoga thermarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
2 h, 98% residual activity Pseudothermotoga thermarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
117
-
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-b-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose pH 6.5, 80°C Pseudothermotoga thermarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Pseudothermotoga thermarum