Any feedback?
Literature summary for 3.2.1.131 extracted from
- Insight into glycoside hydrolases for debranched xylan degradation from extremely thermophilic bacterium Caldicellulosiruptor lactoaceticus (2014), PLoS ONE, 9, e106482.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Caldicellulosiruptor acetigenus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Caldicellulosiruptor acetigenus | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
2 * 80000, SDS-PAGE | Caldicellulosiruptor acetigenus |
| 80343 | - |
2 * 80343, calculated from amino acid sequence | Caldicellulosiruptor acetigenus |
| 163700 | - |
gel filtration | Caldicellulosiruptor acetigenus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | Caldicellulosiruptor acetigenus | - |
4-O-methyl-alpha-D-glucuronic acid + ? | - |
? |  |
| beechwood xylan + H2O | Caldicellulosiruptor acetigenus DSM 9545 | - |
4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor acetigenus | - |
- |
- |
| Caldicellulosiruptor acetigenus DSM 9545 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity column chromatography and Superdex 200 gel filtration | Caldicellulosiruptor acetigenus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldobiouronic acid + H2O | - |
Caldicellulosiruptor acetigenus | 2 D-xylose + 4-O-methyl-D-glucuronic acid | - |
? | |
| aldobiouronic acid + H2O | - |
Caldicellulosiruptor acetigenus DSM 9545 | 2 D-xylose + 4-O-methyl-D-glucuronic acid | - |
? | |
| beechwood xylan + H2O | - |
Caldicellulosiruptor acetigenus | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
| beechwood xylan + H2O | - |
Caldicellulosiruptor acetigenus DSM 9545 | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 80000, SDS-PAGE | Caldicellulosiruptor acetigenus |
| homodimer | 2 * 80343, calculated from amino acid sequence | Caldicellulosiruptor acetigenus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Agu67A | - |
Caldicellulosiruptor acetigenus |
| alpha-glucuronidase | - |
Caldicellulosiruptor acetigenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Caldicellulosiruptor acetigenus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | more than 60% activity between 40 and 90°C | Caldicellulosiruptor acetigenus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Caldicellulosiruptor acetigenus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | more than 50% activity between pH 5.0 and 8.0 | Caldicellulosiruptor acetigenus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
