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Literature summary for 3.2.1.131 extracted from

  • Lee, C.C.; Kibblewhite, R.E.; Wagschal, K.; Li, R.; Robertson, G.H.; Orts, W.J.
    Isolation and characterization of a novel GH67 alpha-glucuronidase from a mixed culture (2012), J. Ind. Microbiol. Biotechnol., 39, 1245-1251.
    View publication on PubMed
Search for more literature for 3.2.1.131

Activating Compound

Activating Compound Comment Organism Structure
xylanase when the enzyme is used in combination with xylanase, a large stimulation in activity occurs uncultured bacterium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysE cells uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ Ca2+ addition results in a slight activity decrease (86.3%) uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the presence of manganese produces higher activity (117.4%) uncultured bacterium
additional information enzyme activity is not dramatically impacted by Co2+, Cu2+, Fe2+, Li+, Mg2+, and Zn2+ at 1 mM uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84500
-
 x * 84500, calculated from amino acid sequence uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
birchwood xylan + H2O uncultured bacterium
-
 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured bacterium I2E7L0
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
 uncultured bacterium 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 84500, calculated from amino acid sequence uncultured bacterium

Synonyms

Synonyms Comment Organism
alpha-glucuronidase
-
 uncultured bacterium
DEG75-AG
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 after 2 h at 40 and 50°C, the enzyme retains most of the initial activity. At 60 °C, half of the activity is lost at 30 min, and no activity remains after 1 h uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
6 9 there is a dramatic decrease in enzyme activity at pH 6.0 and pH 9.0, and there is little activity at pH 4.0 and pH 5.0 uncultured bacterium