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Literature summary for 3.2.1.131 extracted from

  • Rogowski, A.; Basle, A.; Farinas, C.S.; Solovyova, A.; Mortimer, J.C.; Dupree, P.; Gilbert, H.J.; Bolam, D.N.
    Evidence that GH115 alpha-glucuronidase activity, which is required to degrade plant biomass, is dependent on conformational flexibility (2014), J. Biol. Chem., 289, 53-64.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.131

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacteroides ovatus

Crystallization (Commentary)

Crystallization (Comment) Organism
using 19% (w/v) PEG3350, 0.2 M sodium citrate, pH 5.5 Bacteroides ovatus

Protein Variants

Protein Variants Comment Organism
D192A the mutant shows wild type activity Bacteroides ovatus
D206A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
D332A inactive Bacteroides ovatus
D396N the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
D478A the mutant shows wild type activity Bacteroides ovatus
E162A the mutant shows wild type activity Bacteroides ovatus
E375A almost inactive Bacteroides ovatus
E782A the mutant shows reduced activity compared to the wild type enzyme Bacteroides ovatus
E785A the mutant shows reduced activity compared to the wild type enzyme Bacteroides ovatus
H275A the mutant shows severely reduced activity compared to the wild type enzyme Bacteroides ovatus
H275A/H422A inactive Bacteroides ovatus
H422A almost inactive Bacteroides ovatus
K374A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
N205A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
N398A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
N462A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
R328A almost inactive Bacteroides ovatus
W169A the mutant shows wild type activity Bacteroides ovatus
W249A the mutant shows severely reduced activity compared to the wild type enzyme Bacteroides ovatus
Y373A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
Y420A the mutant shows severely reduced activity compared to the wild type enzyme Bacteroides ovatus
Y425A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus
Y788A the mutant shows reduced activity compared to the wild type enzyme Bacteroides ovatus
Y792A the mutant shows strongly reduced activity compared to the wild type enzyme Bacteroides ovatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 birchwood xylan in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
0.4
-
 beechwood xylan in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
4.5
-
 Xyl-beta-(1->4)-[4-O-Me-GlcA-alpha-(1->2)]-Xyl-beta(1->4)-Xyl in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
19.5
-
 4-O-Me-GlcA-alpha-(1->2)-Xyl-beta(1->4)-Xyl in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 2 * 85000, calculated from amino acid sequence Bacteroides ovatus
199000
-
 analytical ultracentrifugation Bacteroides ovatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beechwood xylan + H2O Bacteroides ovatus
-
 4-O-methyl-alpha-D-glucuronic acid + D-glucuronic acid + ?
-
 ?
beechwood xylan + H2O Bacteroides ovatus GH115
-
 4-O-methyl-alpha-D-glucuronic acid + D-glucuronic acid + ?
-
 ?
birchwood xylan + H2O Bacteroides ovatus
-
 4-O-methyl-alpha-D-glucuronic acid + glucuronic acid
-
 ?
birchwood xylan + H2O Bacteroides ovatus GH115
-
 4-O-methyl-alpha-D-glucuronic acid + glucuronic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides ovatus
-
-
-
Bacteroides ovatus GH115
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography and Superdex 75 gel filtration Bacteroides ovatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-Me-GlcA-alpha-(1->2)-Xyl-beta(1->4)-Xyl + H2O
-
 Bacteroides ovatus ?
-
 ?
4-O-Me-GlcA-alpha-(1->2)-Xyl-beta(1->4)-Xyl + H2O
-
 Bacteroides ovatus GH115 ?
-
 ?
beechwood xylan + H2O
-
 Bacteroides ovatus 4-O-methyl-alpha-D-glucuronic acid + D-glucuronic acid + ?
-
 ?
beechwood xylan + H2O
-
 Bacteroides ovatus GH115 4-O-methyl-alpha-D-glucuronic acid + D-glucuronic acid + ?
-
 ?
birchwood xylan + H2O
-
 Bacteroides ovatus 4-O-methyl-alpha-D-glucuronic acid + glucuronic acid
-
 ?
birchwood xylan + H2O
-
 Bacteroides ovatus GH115 4-O-methyl-alpha-D-glucuronic acid + glucuronic acid
-
 ?
glucuronoxylan + H2O
-
 Bacteroides ovatus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
glucuronoxylan + H2O
-
 Bacteroides ovatus GH115 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
glucuronoxylan alpha-(4-O-methyl)-glucuronide + H2O
-
 Bacteroides ovatus xylotriose + xylotetraose + ?
-
 ?
glucuronoxylan alpha-(4-O-methyl)-glucuronide + H2O
-
 Bacteroides ovatus GH115 xylotriose + xylotetraose + ?
-
 ?
Xyl-beta-(1->4)-[4-O-Me-GlcA-alpha-(1->2)]-Xyl-beta(1->4)-Xyl + H2O
-
 Bacteroides ovatus ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 85000, calculated from amino acid sequence Bacteroides ovatus

Synonyms

Synonyms Comment Organism
Agu115A
-
 Bacteroides ovatus
alpha-glucuronidase
-
 Bacteroides ovatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.1
-
 birchwood xylan in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
11.6
-
 beechwood xylan in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
18.4
-
 4-O-Me-GlcA-alpha-(1->2)-Xyl-beta(1->4)-Xyl in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
53.9
-
 Xyl-beta-(1->4)-[4-O-Me-GlcA-alpha-(1->2)]-Xyl-beta(1->4)-Xyl in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacteroides ovatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 4-O-Me-GlcA-alpha-(1->2)-Xyl-beta(1->4)-Xyl in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
12
-
 Xyl-beta-(1->4)-[4-O-Me-GlcA-alpha-(1->2)]-Xyl-beta(1->4)-Xyl in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
29
-
 beechwood xylan in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus
37
-
 birchwood xylan in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C Bacteroides ovatus