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Literature summary for 3.2.1.131 extracted from
- An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening (2009), Appl. Biochem. Biotechnol., 155, 314-320.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | development of an activity assay with commercially available reagents that can be used to search DNA libraries for alpha-glucuronidase genes in a highthroughput, solid phase activity screen | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli SOLR cells carrying pBS expression vectors encoding the alpha-glucuronidase gene | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldouronic acid + H2O | Geobacillus stearothermophilus | - |
? | - |
? |  |
| aldouronic acid + H2O | Geobacillus stearothermophilus T6 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus T6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldobiouronic acid + H2O | - |
Geobacillus stearothermophilus | 2 D-xylose + 4-O-methyl-D-glucuronic acid | - |
? | |
| aldobiouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 2 D-xylose + 4-O-methyl-D-glucuronic acid | - |
? | |
| aldopentaouronic acid + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| aldopentaouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| aldotetraouronic acid + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| aldotetraouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| aldotriouronic acid + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| aldotriouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| aldouronic acid + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| aldouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucuronidase | - |
Geobacillus stearothermophilus |
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