Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.130 extracted from

  • Hakki, Z.; Thompson, A.J.; Bellmaine, S.; Speciale, G.; Davies, G.J.; Williams, S.J.
    Structural and kinetic dissection of the endo-alpha-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp. (2015), Chemistry, 21, 1966-1977.
    View publication on PubMed
Search for more literature for 3.2.1.130

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzyme E333Q, in complex with 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside or alpha-mannopyranosyl-(1->3)-isofagomine, hanging drop vapor diffusion method, using 2.8 M sodium acetate pH 6.4 Bacteroides xylanisolvens

Protein Variants

Protein Variants Comment Organism
E333Q inactive Bacteroides xylanisolvens

Inhibitors

Inhibitors Comment Organism Structure
alpha-mannopyranosyl-(1->3)-isofagomine
-
 Bacteroides xylanisolvens
alpha-mannopyranosyl-1->3-isofagomine
-
 Bacteroides thetaiotaomicron

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
0.404
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
1.827
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron
-
-
-
Bacteroides xylanisolvens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R,4R,5R)-3-(alpha-D-mannopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)-piperidine + H2O
-
 Bacteroides thetaiotaomicron D-mannose + (3R,4R,5R)-3,4-dihydroxy-5-hydroxymethylpiperidine
-
 ?
(alpha-Man-(1->3)-isofagomine) + H2O
-
 Bacteroides xylanisolvens D-mannose + isofagomine
-
 ?
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
alpha-mannan + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
alpha-mannan + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron alpha-(1->3)-D-mannobiose + methyl alpha-(1->2)-D-mannobioside + ?
-
 ?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens alpha-(1->3)-D-mannobiose + methyl alpha-(1->2)-D-mannobioside + ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-alpha-1,2-mannanase
-
 Bacteroides xylanisolvens
endo-alpha-1->2-mannanase
-
 Bacteroides thetaiotaomicron
GH99
-
 Bacteroides thetaiotaomicron
GH99
-
 Bacteroides xylanisolvens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000717
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
0.00008
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
0.00115
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.208
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
1.78
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
6.3
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron
102.7
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron