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Literature summary for 3.2.1.130 extracted from

  • Hiraizumi, S.; Spohr, U.; Spiro, R.G.
    Ligand affinity chromatographic purification of rat liver Golgi endomannosidase (1994), J. Biol. Chem., 269, 4697-4700.
    View publication on PubMed
Search for more literature for 3.2.1.130

Inhibitors

Inhibitors Comment Organism Structure
Glucosyl alpha1-3(1-deoxy)mannojirimycin
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Rattus norvegicus 139
-
membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 ? * 56000 + ? * 60000, 8-10 subunits, SDS-PAGE Rattus norvegicus
60000
-
 ? * 56000 + ? * 60000, 8-10 subunits, SDS-PAGE Rattus norvegicus
560000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcMan9GlcNAc + H2O Rattus norvegicus processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O Rattus norvegicus Cd processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 CD-strain
-
Rattus norvegicus Cd
-
 CD-strain
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity 10.855 units/mg, 1 unit is the amount of enzyme that catalyzes the release of 1000 dpm of glucosyl-alpha-3Man in 1 h Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, in intact Golgi membranes, 10% loss of activity within 6 months Rattus norvegicus
-80°C, purified enzyme, at 0.03 mg protein/ml pH 6.8, 50% loss of activity within 6 months, increased stability at 0.5 mg protein/ml, bovine serum albumin stabilizes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcMan9GlcNAc + H2O
-
 Rattus norvegicus Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units Rattus norvegicus Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O
-
 Rattus norvegicus Cd Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units Rattus norvegicus Cd Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?

Subunits

Subunits Comment Organism
oligomer ? * 56000 + ? * 60000, 8-10 subunits, SDS-PAGE Rattus norvegicus