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Literature summary for 3.2.1.129 extracted from

  • Morley, T.J.; Willis, L.M.; Whitfield, C.; Wakarchuk, W.W.; Withers, S.G.
    A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase (2009), J. Biol. Chem., 284, 17404-17410.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.129

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia phage K1F

Protein Variants

Protein Variants Comment Organism
E581A the mutation results in complete loss of sialidase activity Escherichia phage K1F

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
 trifluoromethylumbelliferyl sialotrioside at pH 4.5 Escherichia phage K1F

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography, gel filtration Escherichia phage K1F

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the endo-sialidase requires the occupation of a minimum of three subsites by sialic acid for efficient catalysis, so neither monomer (trifluoromethylumbelliferyl sialoside) nor dimer (trifluoromethylumbelliferyl sialobioside) are hydrolyzed by endoNF Escherichia phage K1F ?
-
 ?
poly(sialic) acid + H2O
-
 Escherichia phage K1F fragments of poly(sialic) acid
-
 ?
trifluoromethylumbelliferyl sialotetraoside + H2O
-
 Escherichia phage K1F trifluoromethylumbelliferone + sialotetraoside
-
 ?
trifluoromethylumbelliferyl sialotrioside + H2O
-
 Escherichia phage K1F trifluoromethylumbelliferone + sialotrioside
-
 ?

Synonyms

Synonyms Comment Organism
endo-sialidase
-
 Escherichia phage K1F
endoNF endoNF is an inverting sialidase Escherichia phage K1F

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.28
-
 trifluoromethylumbelliferyl sialotrioside at pH 4.5 Escherichia phage K1F

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Escherichia phage K1F