Application | Comment | Organism |
---|---|---|
biotechnology | degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering: no degradation in 12 days with 1 microg/ml active enzyme + 105 cubic mm hydrogel in phosphate buffered saline (pH 7.4), at room temperature, increase to 4 microg/ml at end of week 2 initiates degradation, total degradation after 4 weeks, hydrogel was coated with poly-L-lysine, poly-L-ornithine-laminin or collagen for neurite formation in neonatal and adult rat Schwann cells, neural rat stem cells, and dorsal root ganglionic cells from rats | Escherichia phage K1F |
degradation | degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering: no degradation in 12 days with 1 microg/ml active enzyme + 105 cubic mm hydrogel in phosphate buffered saline (pH 7.4), at room temperature, increase to 4 microg/ml at end of week 2 initiates degradation, total degradation after 4 weeks, hydrogel was coated with poly-L-lysine, poly-L-ornithine-laminin or collagen for neurite formation in neonatal and adult rat Schwann cells, neural rat stem cells, and dorsal root ganglionic cells from rats | Escherichia phage K1F |
medicine | degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering: no degradation in 12 days with 1 microg/ml active enzyme + 105 cubic mm hydrogel in phosphate buffered saline (pH 7.4), at room temperature, increase to 4 microg/ml at end of week 2 initiates degradation, total degradation after 4 weeks, hydrogel was coated with poly-L-lysine, poly-L-ornithine-laminin or collagen for neurite formation in neonatal and adult rat Schwann cells, neural rat stem cells, and dorsal root ganglionic cells from rats | Escherichia phage K1F |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-2,8-linked polysialic acid + H2O | Escherichia phage K1F | specifically cleaves alpha 2,8-linked sialic acid residues with minimum chain length of 8, no effect on other sialic acid containing structures | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | neonatal rat Schwann cell culture (1-3 days postnatal), adult rat Schwann cell culture from 3 months old females, neural stem cells of fetal rat cell culture (gestational day 12), dorsal ganglionic cells of neonatal rat cell culture (2-3 days postnatal) | Escherichia phage K1F | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-2,8-linked polysialic acid + H2O | specifically cleaves alpha 2,8-linked sialic acid residues with minimum chain length of 8, no effect on other sialic acid containing structures | Escherichia phage K1F | ? | - |
? | |
alpha-2,8-linked sialic acid residues cross-linked by diepoxyoctane + H2O | hydrogel with minimum chain length of 8 | Escherichia phage K1F | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-N 1588 | - |
Escherichia phage K1F |
endosialidase | - |
Escherichia phage K1F |