KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
polysialic acid | pH 7.5, capsule isolated from Escherichia coli N67, soluble enzyme | Escherichia phage K1F | |
0.051 | - |
polysialic acid | pH 7.5, capsule isolated from Escherichia coli K1, soluble enzyme | Escherichia phage K1F | |
0.071 | - |
polysialic acid | pH 7.5, capsule isolated from Escherichia coli K1, phage-bound enzyme | Escherichia phage K1F | |
1.2 | - |
oligo(sialic) acid | pH 7.5, isolated from Escherichia coli K1, phage-bound enzyme | Escherichia phage K1F | |
1.6 | - |
oligo(sialic) acid | pH 7.5, from Escherichia coli K1, soluble enzyme | Escherichia phage K1F |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
3 * 105000, SDS-PAGE | Escherichia phage K1F |
328000 | - |
gel filtration | Escherichia phage K1F |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | Escherichia phage K1F | - |
fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia phage K1F |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4219 | - |
- |
Escherichia phage K1F |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligo(sialic) acid + H2O | - |
Escherichia phage K1F | fragments of oligo(sialic) acid | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | - |
Escherichia phage K1F | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | polysialic acid capsule isolated from Escherichia coli N67 or Escherichia coli K1 | Escherichia phage K1F | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | the minimum requirement for cleavage is alpha-2,8-(NeuAc)5 | Escherichia phage K1F | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | bacterial and neural membrane glycoconjugates | Escherichia phage K1F | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | oligosialic acid from Escherichia coli K1 | Escherichia phage K1F | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 105000, SDS-PAGE | Escherichia phage K1F |