Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Phage E |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.41 | - |
K1 antigen | 37°C, pH 6.5, an alpha-2,8-linked homopolymer of N-acetylneuraminic acid | Phage E |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
x * 74000 + x * 38500, SDS-PAGE with boiled samples at 100°C | Phage E |
74000 | - |
x * 74000 + x * 38500, SDS-PAGE with boiled samples at 100°C | Phage E |
208000 | - |
enzyme complex, SDS-PAGE, solubilized in SDS-mercaptoethanol-containing sample buffer at 37°C | Phage E |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | Phage E | - |
fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phage E | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phage E |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.95 | - |
after DEAE-Sephadex chromatography | Phage E |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
K1 antigen | - |
Phage E | ? | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | - |
Phage E | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | no release of alpha-2,3-, alpha-2,6-, or alpha-2,9-linked sialic residues from a variety of other substrates | Phage E | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | limited hydrolysis of the Escherichia coli K92 antigen, an N-acetylneuraminic acid homopolymer containing alternating alpha-2,8-, and alpha-2,9-linkages | Phage E | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? | |
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | rapid hydrolysis of both the O-acetylated and non-O-acetylted forms of the K1 antigen, an alpha-2,8-linked homopolymer of N-acetylneuraminic acid, and of Meningococcus B antigen | Phage E | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 74000 + x * 38500, SDS-PAGE with boiled samples at 100°C | Phage E |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | 5.5 | - |
Phage E |