Cloned (Comment) | Organism |
---|---|
cDNAs encoding BGLU45 and BGLU46 expressed in Pichia pastoris | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
p-coumaryl beta-D-glucoside | BGLU46 | Arabidopsis thaliana | |
5.1 | - |
syringin | BGLU45 | Arabidopsis thaliana | |
7 | - |
coniferin | BGLU45 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity by ammonium sulfate fractionation and hydrophobic interaction chromatography, BGLU45 8.8fold and BGLU46 13.7fold purified | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | BGLU45 and BGLU46 absent from rosette leaves or flowers | Arabidopsis thaliana | - |
root | BGLU46 | Arabidopsis thaliana | - |
silique | BGLU45 | Arabidopsis thaliana | - |
stem | BGLU45 and BGLU46 display increasing levels of expression from apex to base, matching the known increase in lignification | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
BGLU45, crude extract | Arabidopsis thaliana |
35.9 | - |
BGLU45, purified enzyme | Arabidopsis thaliana |
43.8 | - |
BGLU46, crude extract | Arabidopsis thaliana |
599 | - |
BGLU46, purified enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-amygdalin | - |
Arabidopsis thaliana | ? | - |
? | |
4-methylumbelliferyl-beta-D-glucoside | 9% activity with BGLU46 | Arabidopsis thaliana | 4-methylumbelliferol + beta-D-glucose | - |
? | |
arbutin | 6% activity with BGLU46 | Arabidopsis thaliana | benzene-1,4-diol + beta-D-glucose | - |
? | |
coniferin | 87% activity with BGLU45 and 8% activity with BGLU46 | Arabidopsis thaliana | beta-D-glucose + coniferyl aldehyde | - |
? | |
additional information | no activity with sinigrin, methyl-beta-D-glucoside, D(+)cellobiose, beta-gentiobiose, D(+)maltose, sucrose, p-nitrophenyl-1-thio-beta-D-glucopyranoside, p-nitrophenyl-alpha-D-glucopyranoside, p-nitrophenyl-N-acetyl-beta-D-glucosaminide, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-L-fucopyranoside and p-nitrophenyl-beta-L-arabinopyranoside | Arabidopsis thaliana | ? | - |
? | |
o-nitrophenyl-beta-D-glucopyranoside | most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of p-nitrophenyl-beta-D-glucoside degradation | Arabidopsis thaliana | o-nitrophenol + beta-D-glucose | - |
? | |
p-coumaryl beta-D-glucoside | 6.9% activity with BGLU45 and 71% activity with BGLU46 | Arabidopsis thaliana | p-coumaric acid + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside | 0.4% activity with BGLU45 and 21% activity with BGLU46 | Arabidopsis thaliana | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside | 100% activity with BGLU45 and BGLU46 | Arabidopsis thaliana | p-nitrophenol + beta-D-glucose | - |
? | |
phenyl-beta-D-glucoside | 62% activity with BGLU46 | Arabidopsis thaliana | phenol + beta-D-glucose | - |
? | |
salicin | 100% activity with BGLU46 | Arabidopsis thaliana | beta-D-glucose + salicyl alcohol | - |
? | |
syringin + H2O | 100% activity with BGLU45 and 6% activity with BGLU46 | Arabidopsis thaliana | D-glucose + sinapyl alcohol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Arabidopsis thaliana |
BGLU45 | - |
Arabidopsis thaliana |
BGLU46 | - |
Arabidopsis thaliana |