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Literature summary for 3.2.1.126 extracted from
- Agrobacterium tumefaciens beta-glucosidase is also an effective beta-xylosidase, and has a high transglycosylation activity in the presence of alcohols (1998), Biochim. Biophys. Acta, 1385, 78-88.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | 20 mM, activates due to transglycosylation activity with over 80% of p-nitrophenyl beta-D-glucopyranoside being converted to 1-butyl beta-D-glucopyranoside in the presence of Cbg1 and 100 mM 1-butanol | Agrobacterium tumefaciens |  |
| 1-Heptanol | 20 mM, activates | Agrobacterium tumefaciens | |
| 1-Hexanol | 20 mM, activates | Agrobacterium tumefaciens | |
| 1-Octanol | 20 mM, activates | Agrobacterium tumefaciens | |
| 1-Pentanol | 20 mM, activates | Agrobacterium tumefaciens | |
| 1-propanol | 20 mM, activates, highest rate of consumption of p-nitrophenyl beta-D-glucopyranoside in the presence of 0.6 M 1-propanol | Agrobacterium tumefaciens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Agrobacterium tumefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
| deoxynojirimycin | strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
| glucuronolactone | strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
| isopropyl 1-thio-beta-D-glucopyranoside | competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
| additional information | not inhibited by 24 mM xylose | Agrobacterium tumefaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Agrobacterium tumefaciens | |
| 0.005 | - |
p-nitrophenyl beta-D-xylopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.012 | - |
p-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.021 | - |
arbutin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.023 | - |
coniferin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.045 | - |
Salicin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.06 | - |
o-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.08 | - |
p-nitrophenyl beta-D-fucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.27 | - |
p-nitrophenyl alpha-L-arabinofuranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 4 | - |
p-nitrophenyl beta-D-galactopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88289 | - |
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation | Agrobacterium tumefaciens |
| 98000 | - |
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation | Agrobacterium tumefaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferin + H2O | Agrobacterium tumefaciens | natural substrate | D-glucose + coniferol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Cbg1 | Agrobacterium tumefaciens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| coniferin + H2O = D-glucose + coniferol | mechanism | Agrobacterium tumefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 39 | 41 | pH 6.5, 30°C | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arbutin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
| coniferin + H2O | natural substrate | Agrobacterium tumefaciens | D-glucose + coniferol | - |
? | |
| coniferin + H2O | 20000fold preference for the natural substrate coniferin over cellobiose | Agrobacterium tumefaciens | D-glucose + coniferol | - |
? | |
| additional information | enzyme has a high transglycosylation activity in the presence of alcohols, transglycosylation mechanism | Agrobacterium tumefaciens | ? | - |
? | |
| o-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | beta-D-glucose + o-nitrophenol | - |
? | |
| p-nitrophenyl alpha-L-arabinofuranoside + H2O | with moderate efficiency | Agrobacterium tumefaciens | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| p-nitrophenyl beta-cellobioside + H2O | releases only terminal glucose from the substrate | Agrobacterium tumefaciens | ? | - |
? | |
| p-nitrophenyl beta-D-fucopyranoside + H2O | with moderate efficiency | Agrobacterium tumefaciens | beta-D-fucose + p-nitrophenol | - |
? | |
| p-nitrophenyl beta-D-galactopyranoside + H2O | with low efficiency | Agrobacterium tumefaciens | beta-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | beta-D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | 73% of the efficiency with p-nitrophenyl beta-D-glucopyranoside | Agrobacterium tumefaciens | beta-D-xylose + p-nitrophenol | - |
? | |
| salicin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 98000, about, SDS-PAGE, x * 88289, sequence calculation | Agrobacterium tumefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cbg1 | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Agrobacterium tumefaciens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Agrobacterium tumefaciens | |
| 3.15 | - |
Salicin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 3.2 | - |
p-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 22.1 | - |
p-nitrophenyl beta-D-fucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 28.9 | - |
p-nitrophenyl beta-D-xylopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 46.6 | - |
p-nitrophenyl beta-D-galactopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 86.7 | - |
Salicin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 95.4 | - |
p-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 106 | - |
coniferin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 118 | - |
p-nitrophenyl alpha-L-arabinofuranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 155 | - |
o-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Agrobacterium tumefaciens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00078 | - |
deoxynojirimycin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 0.0039 | - |
glucuronolactone | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 2.9 | - |
D-glucose | pH 6.5, 30°C | Agrobacterium tumefaciens | |
| 5.6 | - |
isopropyl 1-thio-beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Agrobacterium tumefaciens | isoelectric focusing, pH gradient 4-7 | - |
4.5 |
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