Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzyme E186Q in complex with the substrate 1,2-(S)-dihydroraucaffricine and secologanin, hanging drop vapor diffusion method, using 0.01-0.3 M ammonium sulfate, 0.1 M sodium acetate, pH 4.5-5.0, 9-12% (w/v) PEG 4000 as precipitant buffer, and 20°C | Rauvolfia serpentina |
Protein Variants | Comment | Organism |
---|---|---|
E186D | inactive | Rauvolfia serpentina |
E186Q | inactive | Rauvolfia serpentina |
E186Q/E420Q | inactive | Rauvolfia serpentina |
E420Q | the mutant shows 0.5% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
E476A | the mutant shows 0.57% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
E476L | the mutant shows 0.67% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
F485Y | the mutant shows 24.24% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
H193A | the mutant shows 43.66% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
S390G | the mutant shows 30.91% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
T189A | the mutant shows 63.94% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
W392A | the mutant shows 1.21% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
Y200A | the mutant shows 4.6% activity with raucaffricine compared to the wild type enzyme | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Raucaffricine | wild type enzyme, pH and temperature not specified in the publication | Rauvolfia serpentina | |
1.8 | - |
strictosidine | wild type enzyme, pH and temperature not specified in the publication | Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-(S)-dihydro-raucaffricine + H2O | 98% acivity compared to raucaffricine | Rauvolfia serpentina | 2beta(R)-1,2-dihydrovomilenine + D-glucose | - |
? | |
additional information | no activity with arbutin | Rauvolfia serpentina | ? | - |
? | |
raucaffricine + H2O | 100% activity | Rauvolfia serpentina | D-glucose + vomilenine | - |
? | |
secologanin + H2O | 0.24% acivity compared to raucaffricine | Rauvolfia serpentina | secologanin aglycone | - |
? | |
strictosidine + H2O | 1.18% acivity compared to raucaffricine | Rauvolfia serpentina | strictosidine aglycone + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
raucaffricine glucosidase | - |
Rauvolfia serpentina |
raucaffricine O-beta-D-glucosidase | - |
Rauvolfia serpentina |