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Literature summary for 3.2.1.123 extracted from

  • Li, Y.T.; Chou, C.W.; Li, S.C.; Kobayashi, U.; Ishibashi, Y.H.; Ito, M.
    Preparation of homogenous oligosaccharide chains from glycosphingolipids (2009), Glycoconj. J., 26, 929-933.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information for maximal hydrolysis of glycosphingolipids by rhodococcal EGCases, a detergent such as Triton X-100 is required at a final concentration of 0.1-0.3% of the reaction mixture. The hydrolysis of water soluble gangliosides such as GM1 does not require the presence of a detergent. For the hydrolysis of GM1, the addition of 5% tetrahydrofuran instead of Triton X-100 increases the reaction rate of EGCase I by 2fold compared to that in the absence of a detergent. Tetrahydrofuran is ineffective in facilitating the hydrolysis of GbOse4Cer by EGCase I. DMSO, ethyl acetate, and methyl cellosolve are also not effective in stimulating the hydrolysis of GM1 or GbOse4Cer by EGCase I in the absence of a detergent Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. M-750
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ganglioside fucosyl-GM1 + H2O 100% hydrolyzed by EGCase I and 19% hydrolyzed by EGCase II Rhodococcus sp. ?
-
?
ganglioside GbOse3Cer + H2O 73% hydrolyzed by EGCase I and 24% hydrolyzed by EGCase II Rhodococcus sp. ?
-
?
ganglioside GbOse4Cer + H2O 34% hydrolyzed by EGCase I and 7% hydrolyzed by EGCase II Rhodococcus sp. ?
-
?
ganglioside GbOse5Cer + H2O 25% hydrolyzed by EGCase I and 6.5% hydrolyzed by EGCase II Rhodococcus sp. ?
-
?
ganglioside GD1a + H2O 100% hydrolyzed by EGCase I and II Rhodococcus sp. ?
-
?
ganglioside GD1a + H2O 100% hydrolyzed by EGCase I and II Rhodococcus sp. M-750 ?
-
?
ganglioside GD1b + H2O 87% hydrolyzed by EGCase I and 74% hydrolyzed by EGCase II Rhodococcus sp. ?
-
?
ganglioside GD1b + H2O 87% hydrolyzed by EGCase I and 74% hydrolyzed by EGCase II Rhodococcus sp. M-750 ?
-
?
ganglioside GD3 + H2O 100% hydrolyzed by EGCase I and II Rhodococcus sp. ?
-
?
ganglioside GD3 + H2O 100% hydrolyzed by EGCase I and II Rhodococcus sp. M-750 ?
-
?
ganglioside GM1 + H2O 100% hydrolyzed by EGCase I and II Rhodococcus sp. ?
-
?
ganglioside GM1 + H2O 100% hydrolyzed by EGCase I and II Rhodococcus sp. M-750 ?
-
?
ganglioside TGC + H2O 66% hydrolyzed by EGCase III Rhodococcus sp. ?
-
?
additional information both rhodococcal EGCases I and II efficiently hydrolyze ganglio-series glycosphingolipids, whereas EGCase I hydrolyzes globo-series-glycosphingolipids much faster than EGCase II. The glycan chain from fucosyl-GM1 can be prepared by using EGCase I. EGCase III stands alone to hydrolyze 6-gala-series glycosphingolipids. All EGCases are not able to hydrolyze sphingomyelin and sulfatide Rhodococcus sp. ?
-
?
additional information both rhodococcal EGCases I and II efficiently hydrolyze ganglio-series glycosphingolipids, whereas EGCase I hydrolyzes globo-series-glycosphingolipids much faster than EGCase II. The glycan chain from fucosyl-GM1 can be prepared by using EGCase I. EGCase III stands alone to hydrolyze 6-gala-series glycosphingolipids. All EGCases are not able to hydrolyze sphingomyelin and sulfatide Rhodococcus sp. M-750 ?
-
?

Synonyms

Synonyms Comment Organism
EGCase I
-
Rhodococcus sp.
EGCase II
-
Rhodococcus sp.
EGCase III
-
Rhodococcus sp.