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Literature summary for 3.2.1.123 extracted from

  • Ito, M.; Ikegami, Y.; Yamagata, T.
    Activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases. Elucidation of biological functions of cell-surface glycosphingolipids in situ by endoglycoceramidases made possible using these activator proteins (1991), J. Biol. Chem., 266, 7919-7926.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
Activator protein purified from culture supernatant of Rhodococcus sp. M-777, enhances the hydrolysis of glycosphingolipids in vitro and on the cell-surface by EGCase in absence of detergent Rhodococcus sp.
detergent required Rhodococcus sp.

General Stability

General Stability Organism
highly purified ECGase II loses activity due to instability following purification Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
glycosphingolipid + H2O GM3 Rhodococcus sp. ?
-
 ?
glycosphingolipid + H2O GM2 Rhodococcus sp. ?
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 isoform II, without activator protein Rhodococcus sp.
5.5
-
 isoform II, with activator protein Rhodococcus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Rhodococcus sp. pH gradient from pH 3 to pH 10
-
 4