Activating Compound | Comment | Organism | Structure |
---|---|---|---|
taurodeoxycholate | 0.5 mg/ml, 2.5fold increase of activity | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Rhodococcus sp. | |
Hg2+ | - |
Rhodococcus sp. | |
Zn2+ | - |
Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
G-74-2 | - |
Rhodococcus sp. G-74-2 | - |
G-74-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Rhodococcus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glcbeta(1-1)ceramide + H2O | 84% of the activity with galactosylcerebroside | Rhodococcus sp. | Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM1 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | as active as lactosylceramide | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM1 | Rhodococcus sp. G-74-2 | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | as active as lactosylceramide | Rhodococcus sp. G-74-2 | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | as active as lactosylceramide | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. asialo-GM1 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. lactosylceramide | Rhodococcus sp. | Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-6)Galbeta(1-6)Galbeta(1-1)ceramide + H2O | i.e. neogalactosylceramide, 77% of the activity with lactosylceramide | Rhodococcus sp. | Galbeta(1-6)Galbeta(1-6)Gal + ceramide | - |
? | |
GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GM2, 94% of the activity with lactosylceramide | Rhodococcus sp. | GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O | 35% of the activity with lactosylceramide | Rhodococcus sp. | GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glc + ceramide | - |
? | |
Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O | 88% of the activity with lactosylceramide | Rhodococcus sp. | Manalpha(1-3)Manbeta(1-4)Glc + ceramide | - |
? | |
Manbeta(1-4)Glcbeta(1-1)ceramide + H2O | 93% of the activity with lactosylceramide | Rhodococcus sp. | Manbeta(1-4)Glc + ceramide | - |
? | |
additional information | no substrate: N-linked glycoproteins | Rhodococcus sp. | ? | - |
? | |
additional information | no substrate: O-linked glycoproteins | Rhodococcus sp. | ? | - |
? | |
additional information | hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids | Rhodococcus sp. | ? | - |
? | |
additional information | no substrate: N-linked glycoproteins | Rhodococcus sp. G-74-2 | ? | - |
? | |
additional information | no substrate: O-linked glycoproteins | Rhodococcus sp. G-74-2 | ? | - |
? | |
additional information | hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids | Rhodococcus sp. G-74-2 | ? | - |
? | |
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GD, 93% of the activity with lactosylceramide | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GT1b | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | 93% of the activity with lactosylceramide | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM3 | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. sialylparagloboside, as active as lactosylceramide | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GT, 81% of the activity with lactosylceramide | Rhodococcus sp. | NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rhodococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | stable | Rhodococcus sp. |