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Literature summary for 3.2.1.122 extracted from

  • Thompson, J.; Hess, S.; Pikis, A.
    Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s) (2004), J. Biol. Chem., 279, 1553-1561.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol dithiothreitol-dependent Clostridium acetobutylicum
additional information differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL Clostridium acetobutylicum

Cloned(Commentary)

Cloned (Comment) Organism
genes malh and pagl encoding maltose 6’-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl Clostridium acetobutylicum

Protein Variants

Protein Variants Comment Organism
C169S catalytically inactive MalH mutant Clostridium acetobutylicum
D170N catalytically inactive MalH mutant Clostridium acetobutylicum
M171V MalH mutant with reduced activity Clostridium acetobutylicum
P172A MalH mutant with reduced activity Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate pH 7, 37°C Clostridium acetobutylicum
0.058
-
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate pH 7, 37°C Clostridium acetobutylicum
0.82
-
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose pH 7, 37°C Clostridium acetobutylicum
1.11
-
maltulose 6'-phosphate pH 7, 37°C Clostridium acetobutylicum
1.87
-
leucrose 6'-phosphate pH 7, 37°C Clostridium acetobutylicum
1.92
-
trehalulose 6'-phosphate pH 7, 37°C Clostridium acetobutylicum
1.95
-
maltose 6'-phosphate pH 7, 37°C Clostridium acetobutylicum
2.47
-
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose pH 7, 37°C Clostridium acetobutylicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ cofactor, requirement Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49972
-
4 * 49972, recombinant MalH, predicted from the amino acid sequence Clostridium acetobutylicum
50000
-
4 * 50000, recombinant MalH and PagL, SDS-PAGE Clostridium acetobutylicum
50038
-
4 * 50038, recombinant MalH, electrospray ionization mass spectrometry Clostridium acetobutylicum
51155
-
4 * 51155, recombinant PagL, predicted from the amino acid sequence Clostridium acetobutylicum
51205
-
4 * 51205, recombinant PagL, electrospray ionization mass spectrometry Clostridium acetobutylicum
200000
-
about, MalH and PagL, HPLC gel filtration Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium acetobutylicum MalH participates in dissimilation of maltose and sucrose isomers, PagL not ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum Q97DP6 strain ATCC 824, PagL, i.e. phospho-alpha-glucosidase
-
Clostridium acetobutylicum Q97LM4 strain ATCC 824, MalH, i.e. maltose 6’-phosphate hydrolase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MalH expressed in Escherichia coli PEP43 Clostridium acetobutylicum
recombinant PagL expressed in Escherichia coli PEP43 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
pH 7.5, 37°C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
-
Clostridium acetobutylicum alpha-D-glucose 6-phosphate + 4-methylumbelliferone
-
?
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
-
Clostridium acetobutylicum ?
-
?
leucrose 6'-phosphate + H2O
-
Clostridium acetobutylicum D-glucose 6-phosphate + D-fructose
-
?
maltose 6'-phosphate + H2O
-
Clostridium acetobutylicum D-glucose 6-phosphate + D-glucose
-
?
maltulose 6'-phosphate + H2O
-
Clostridium acetobutylicum D-glucose 6-phosphate + D-fructose
-
?
additional information not: methyl-beta-glucose 6-phosphate Clostridium acetobutylicum ?
-
?
additional information not: cellobiose 6’-phosphate, gentiobiose 6’-phosphate Clostridium acetobutylicum ?
-
?
additional information only MalH catalyzes the hydrolysis of disaccharide 6’-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL Clostridium acetobutylicum ?
-
?
additional information substrate specificity, not: sucrose 6-phosphate Clostridium acetobutylicum ?
-
?
additional information MalH participates in dissimilation of maltose and sucrose isomers, PagL not Clostridium acetobutylicum ?
-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O MalH and PagL, Cys-169 and Asp-170 are essential for hydrolysis Clostridium acetobutylicum alpha-D-glucose 6-phosphate + p-nitrophenol yellow product ?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O only substrate Clostridium acetobutylicum alpha-D-glucose 6-phosphate + p-nitrophenol yellow product ?
palatinose 6'-phosphate + H2O
-
Clostridium acetobutylicum D-glucose 6-phosphate + D-fructose
-
?
trehalulose 6'-phosphate + H2O
-
Clostridium acetobutylicum alpha-D-glucose 6-phosphate + D-fructose
-
?
turanose 6'-phosphate + H2O
-
Clostridium acetobutylicum D-glucose 6-phosphate + D-fructose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 51155, recombinant PagL, predicted from the amino acid sequence Clostridium acetobutylicum
tetramer 4 * 49972, recombinant MalH, predicted from the amino acid sequence Clostridium acetobutylicum
tetramer 4 * 50038, recombinant MalH, electrospray ionization mass spectrometry Clostridium acetobutylicum
tetramer 4 * 51205, recombinant PagL, electrospray ionization mass spectrometry Clostridium acetobutylicum
tetramer 4 * 50000, recombinant MalH and PagL, SDS-PAGE Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
MalH
-
Clostridium acetobutylicum
More belongs to familiy 4 of the glycosylhydrolase superfamily Clostridium acetobutylicum
PagL
-
Clostridium acetobutylicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Clostridium acetobutylicum

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+-dependent Clostridium acetobutylicum