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Literature summary for 3.2.1.121 extracted from
- Partial purification and characterization of a polymannuronic acid depolymerase produced by a mycoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis (1985), Appl. Environ. Microbiol., 50, 562-567.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | at 17 mM inhibition | Pseudomonas aeruginosa |  |
| EDTA | 0.5 mM: 35% inhibition | Pseudomonas aeruginosa | |
| KCl | 50 mM: 50% inhibition | Pseudomonas aeruginosa | |
| MgCl2 | at 9 mM inhibition | Pseudomonas aeruginosa | |
| MgSO4 | at 14 mM inhibition | Pseudomonas aeruginosa | |
| NaCl | 50 mM: 40% inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.096 | - |
polymannuronic acid exopolysaccharide | 37°C, pH 7.5, from Pseudomonas aeruginosa, strain PM1 | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
| membrane | associated with outer cell membrane | Pseudomonas aeruginosa | 16020 | - |
| periplasm | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 5 mM: slight stimulation, at 17 mM inhibition | Pseudomonas aeruginosa | |
| KCl | 3 mM: stimulation, above 10 mM: inhibition | Pseudomonas aeruginosa | |
| MgCl2 | 3 mM: slight stimulation, at 9 mM inhibition | Pseudomonas aeruginosa | |
| MgSO4 | 3 mM: slight stimulation, at 14 mM inhibition | Pseudomonas aeruginosa | |
| NaCl | 3 mM: stimulation, above 10 mM: inhibition | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polymannuronic acid exopolysaccharide + H2O | Pseudomonas aeruginosa | from Pseudomonas aeruginosa, strain PM1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.8 | - |
sonicated cells with EDTA | Pseudomonas aeruginosa |
| 2 | - |
sonicated cells without EDTA | Pseudomonas aeruginosa |
| 3.8 | - |
supernatant of cells with EDTA | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginic acid + H2O | little activity | Pseudomonas aeruginosa | ? | - |
? | |
| polymannuronic acid exopolysaccharide + H2O | from Pseudomonas aeruginosa, strain PM1 | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | major band: 53000 Da, minor band: 23000 Da, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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