Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST fusion protein, full-length cDNA subcloned into the pFastBac1 plasmid for expression in insect cells using a baculovirus expression system | Vicia sativa subsp. nigra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55616 | - |
1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis | Vicia sativa subsp. nigra |
56000 | - |
1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis | Vicia sativa subsp. nigra |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vicia sativa subsp. nigra | A2SY66 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, 9.39fold | Vicia sativa subsp. nigra |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | moderately present | Vicia sativa subsp. nigra | - |
leaf | slightly present | Vicia sativa subsp. nigra | - |
root | slightly present | Vicia sativa subsp. nigra | - |
seed | abundantly present | Vicia sativa subsp. nigra | - |
stem | slightly present | Vicia sativa subsp. nigra | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
crude extract | Vicia sativa subsp. nigra |
8.97 | - |
recombinant enzyme, activity against vicianin | Vicia sativa subsp. nigra |
9.86 | - |
native enzyme, purified, activity against vicianin | Vicia sativa subsp. nigra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethyl beta-primeveroside + H2O | - |
Vicia sativa subsp. nigra | ? | - |
? | |
furcatin + H2O | recombinant enzyme expressed in Escherichia coli does not hydrolyze furcatin | Vicia sativa subsp. nigra | ? | - |
? | |
additional information | does not hydrolyze amygdalin, mandelonitrile-, 2-phenylethyl-, p-nitrophenyl beta-gentiobioside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl alpha-L-arabinopyranoside and p-nitrophenyl beta-D-galactopyranoside | Vicia sativa subsp. nigra | ? | - |
? | |
p-nitrophenyl beta-primeveroside + H2O | recombinant enzyme expressed in Escherichia coli does not hydrolyze p-nitrophenyl beta-primeveroside | Vicia sativa subsp. nigra | ? | - |
? | |
prunasin + H2O | shows 34% of its vicianin hydrolase activity with prunasin | Vicia sativa subsp. nigra | D-mandelonitrile + beta-D-glucose | - |
? | |
vicianin + H2O | best substrate, recombinant protein shows the same pattern of substrate specificity as the native enzyme | Vicia sativa subsp. nigra | vicianose + D-mandelonitrile | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis | Vicia sativa subsp. nigra |
Synonyms | Comment | Organism |
---|---|---|
vicianin hydrolase | - |
Vicia sativa subsp. nigra |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vicia sativa subsp. nigra | - |
- |
8.69 |