Application | Comment | Organism |
---|---|---|
diagnostics | evaluating intestinal absorption of amygdalin or prunasin, by estimating its transfer across the mucosal border as well as its uptake into the intestinal tissue, for judgement of toxication rusks, overview. Use of the enzyme in an in vitro digestion model with the Caco-2 cell to estimate the human oral bioavailability of cyanogenic compounds from food or plants, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prunasin + H2O | Homo sapiens | prunasin is taken up in the small intestine and decomposed by beta-glucosidase into glucose and mandelonitrile, which is further hydrolyzed to benzaldehyde and hydrogen cyanide. Amygdalin, the precursor of prunasin, is a toxic component and can cause fatal cyanide poisonings, chronic toxicity, and death | D-mandelonitrile + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Caco-2 cell | TC7 clone | Homo sapiens | - |
digestive juice | - |
Homo sapiens | - |
small intestine | use of a simulated in vitro salivary-gastric-small intestinal digestion model system | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prunasin + H2O | prunasin is taken up in the small intestine and decomposed by beta-glucosidase into glucose and mandelonitrile, which is further hydrolyzed to benzaldehyde and hydrogen cyanide. Amygdalin, the precursor of prunasin, is a toxic component and can cause fatal cyanide poisonings, chronic toxicity, and death | Homo sapiens | D-mandelonitrile + beta-D-glucose | - |
? | |
prunasin + H2O | substrate and product determination in the in vitro system by mass spectrometry, method, overview | Homo sapiens | D-mandelonitrile + beta-D-glucose | - |
? |