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Literature summary for 3.2.1.116 extracted from

  • Lee, Y.S.; Park, D.J.; Choi, Y.L.
    Characterization of maltotriose production by hydrolyzing of soluble starch with alpha-amylase from Microbulbifer thermotolerans DAU221 (2015), Appl. Microbiol. Biotechnol., 99, 3901-3911.
    View publication on PubMed
Search for more literature for 3.2.1.116

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Microbulbifer thermotolerans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 53% residual activity at 10 mM Microbulbifer thermotolerans
Cu2+ 19% residual activity at 10 mM Microbulbifer thermotolerans
EDTA 23% residual activity at 10 mM Microbulbifer thermotolerans
Fe3+ 19% residual activity at 10 mM Microbulbifer thermotolerans
Hg2+ 17% residual activity at 10 mM Microbulbifer thermotolerans
Ni2+ 77% residual activity at 10 mM Microbulbifer thermotolerans
Zn2+ 18% residual activity at 10 mM Microbulbifer thermotolerans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Mg2+, Ca2+, Cs2+, Li+, and Mn2+ Microbulbifer thermotolerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Microbulbifer thermotolerans

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 88% activity at 20% (v/v) Microbulbifer thermotolerans
benzene 93% activity at 20% (v/v) Microbulbifer thermotolerans
chloroform 29% activity at 20% (v/v) Microbulbifer thermotolerans
Ethanol 61% activity at 20% (v/v) Microbulbifer thermotolerans
hexane the enzyme exhibits extreme stability toward 20% (v/v) hexane (99% activity) Microbulbifer thermotolerans
Methanol 56% activity at 20% (v/v) Microbulbifer thermotolerans
n-Butanol 51% activity at 20% (v/v) Microbulbifer thermotolerans

Organism

Organism UniProt Comment Textmining
Microbulbifer thermotolerans A0A0A0Q4S7
-
-
Microbulbifer thermotolerans DAU221 A0A0A0Q4S7
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag affinity column chromatography Microbulbifer thermotolerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O the Km and Vmax values for the hydrolysis of soluble starch are 1.08 mg/ml and 1.736 mM maltotriose/mg protein/min, respectively Microbulbifer thermotolerans maltotriose + ?
-
 ?
soluble starch + H2O the Km and Vmax values for the hydrolysis of soluble starch are 1.08 mg/ml and 1.736 mM maltotriose/mg protein/min, respectively Microbulbifer thermotolerans DAU221 maltotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Microbulbifer thermotolerans

Synonyms

Synonyms Comment Organism
AmyA
-
 Microbulbifer thermotolerans
maltotriose-producing alpha-amylase
-
 Microbulbifer thermotolerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in sodium phosphate buffer (pH 6.0) Microbulbifer thermotolerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 at 30°C, the enzyme maintains approximately above 90% activity until 6 h and 80% activity until 12 h. At 40°C, the enzyme maintains approximately above 90% activity until 2 h, 80% activity until 4 h, 70% activity until 8 h, and 60% activity until 12 h. At 50°C, the enzyme maintains approximately 50% activity until 1 h, 30% activity until 6 h, and 20% activity until 12 h Microbulbifer thermotolerans